EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.55 | expressed in Escherichia coli BL21(DE3) cells | Acetivibrio clariflavus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | alpha-L-arabinose | the enzyme retains 80.6% activity in the presence of 50 mM and 55.3% activity in the presence of 167 mM | Acetivibrio clariflavus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 3.08 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 8.01 | - |
2-alpha-L-arabinofuranosyl xylotriose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 32.6 | - |
3-alpha-L-arabinofuranosyl xylobiose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 34.4 | - |
2,3-di-alpha-L-arabinofuranosyl xylotriose | at pH 6.5 and 60°C | Acetivibrio clariflavus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.55 | Acetivibrio clariflavus | - |
- |
- |
3.2.1.55 | Acetivibrio clariflavus DSM 19732 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.55 | nickel column chromatography | Acetivibrio clariflavus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.55 | 2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O | - |
Acetivibrio clariflavus | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | 2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O | - |
Acetivibrio clariflavus DSM 19732 | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | 2-alpha-L-arabinofuranosyl xylotriose + H2O | - |
Acetivibrio clariflavus | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | 2-alpha-L-arabinofuranosyl xylotriose + H2O | - |
Acetivibrio clariflavus DSM 19732 | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | 3-alpha-L-arabinofuranosyl xylobiose + H2O | - |
Acetivibrio clariflavus | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | 3-alpha-L-arabinofuranosyl xylobiose + H2O | - |
Acetivibrio clariflavus DSM 19732 | alpha-L-arabinose + ? | - |
? | |
3.2.1.55 | 4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Acetivibrio clariflavus | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
3.2.1.55 | 4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Acetivibrio clariflavus DSM 19732 | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
3.2.1.55 | additional information | no activity with 4-nitrophenyl beta-D-xylopyranoside | Acetivibrio clariflavus | ? | - |
? | |
3.2.1.55 | additional information | no activity with 4-nitrophenyl beta-D-xylopyranoside | Acetivibrio clariflavus DSM 19732 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.55 | ? | x * 56000, SDS-PAGE | Acetivibrio clariflavus |
3.2.1.55 | ? | x * 56000, calculated from amino acid sequence | Acetivibrio clariflavus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.55 | AbF51 | - |
Acetivibrio clariflavus |
3.2.1.55 | GH51 alpha-arabinofuranosidase | - |
Acetivibrio clariflavus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 60 | - |
- |
Acetivibrio clariflavus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 45 | 70 | more than 60% activity between 45 and 70°C | Acetivibrio clariflavus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 60 | 70 | at 60°C, the enzyme retains over 90% activity after a 2-day incubation and over 60% activity after a 6-day incubation. The enzyme remains approximately 58% activity after a 1-day incubation at 70°C | Acetivibrio clariflavus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 15.9 | - |
2,3-di-alpha-L-arabinofuranosyl xylotriose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 55.2 | - |
2-alpha-L-arabinofuranosyl xylotriose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 631.4 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 743.9 | - |
3-alpha-L-arabinofuranosyl xylobiose | at pH 6.5 and 60°C | Acetivibrio clariflavus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 6.5 | - |
- |
Acetivibrio clariflavus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 200 | - |
at pH 6.5 and 60°C | Acetivibrio clariflavus | alpha-L-arabinose |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 0.5 | - |
2,3-di-alpha-L-arabinofuranosyl xylotriose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 6.9 | - |
2-alpha-L-arabinofuranosyl xylotriose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 22.8 | - |
3-alpha-L-arabinofuranosyl xylobiose | at pH 6.5 and 60°C | Acetivibrio clariflavus | |
3.2.1.55 | 205 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 6.5 and 60°C | Acetivibrio clariflavus |