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Literature summary extracted from

  • Varriale, S.; Cerullo, G.; Antonopoulou, I.; Christakopoulos, P.; Rova, U.; Tron, T.; Faure, R.; Juetten, P.; Piechot, A.; Bras, J.L.A.; Fontes, C.M.G.A.; Faraco, V.
    Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis (2018), Appl. Microbiol. Biotechnol., 102, 5185-5196 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Saccharomyces cerevisiae Thermothelomyces thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 molecular docking of methyl hydroxycinnamates to wild-type and mutant structures Thermothelomyces thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 F251L/H105Y mutant displays improved activity and thermostability Thermothelomyces thermophilus
3.1.1.73 G49A mutant displays improved activity and thermostability Thermothelomyces thermophilus
3.1.1.73 G49D mutant displays improved activity and thermostability Thermothelomyces thermophilus
3.1.1.73 H105Y mutant displays improved activity and thermostability Thermothelomyces thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.73 Acetone wild-type, 25% acetone, v/v, 1 h, 67% residual activity Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Thermothelomyces thermophilus G2QND5
-
-
3.1.1.73 Thermothelomyces thermophilus DSM 1799 G2QND5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrocatechol-1-yl ferulate + H2O
-
 Thermothelomyces thermophilus 4-nitrocatechol + ferulate
-
 ?
3.1.1.73 4-nitrocatechol-1-yl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 4-nitrocatechol + ferulate
-
 ?
3.1.1.73 4-nitrophenyl ferulate + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + ferulate
-
 ?
3.1.1.73 4-nitrophenyl ferulate + H2O
-
 Thermothelomyces thermophilus DSM 1799 4-nitrophenol + ferulate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Fae1A
-
 Thermothelomyces thermophilus
3.1.1.73 feruloyl esterase B
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 55
-
 wild-type, 1 h, 10.8%, mutant H105Y 34.3%, mutant F251L/H105Y 21.4%, mutant G49D 46.2%, mutant G49A 37.4% residual activity, respectively Thermothelomyces thermophilus