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Literature summary extracted from

  • Ma, M.; Okuyama, M.; Sato, M.; Tagami, T.; Klahan, P.; Kumagai, Y.; Mori, H.; Kimura, A.
    Effects of mutation of Asn694 in Aspergillus niger alpha-glucosidase on hydrolysis and transglucosylation (2017), Appl. Microbiol. Biotechnol., 101, 6399-6408 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 gene Agl, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Pichia pastoris Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.20 N694A site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type Aspergillus niger
3.2.1.20 N694F site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type, the pH optimum is increased compared to the wild-type enzyme Aspergillus niger
3.2.1.20 N694L site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type, the pH optimum is increased and the thermal stability slightly reduced compared to the wild-type enzyme Aspergillus niger
3.2.1.20 N694W site-directed mutagenesis, the mutant shows altered transglucosylation activity compared to the wild-type Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information kinetics for transglucosylation of wild-type and mutant enzymes, overview Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Aspergillus niger A0PCH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant His-tagged wild-type and mutant enzymes from Pichia pastoris by nickel affinity chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 120
-
 enzyme mutant N694L, transglucosylation activity, pH 4.0, 37°C Aspergillus niger
3.2.1.20 152
-
 enzyme mutant N694A, transglucosylation activity, pH 4.0, 37°C Aspergillus niger
3.2.1.20 155
-
 enzyme mutant N694W, transglucosylation activity, pH 4.0, 37°C Aspergillus niger
3.2.1.20 185
-
 enzyme mutant N694F, transglucosylation activity, pH 4.0, 37°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 maltose + H2O
-
 Aspergillus niger 2 D-glucose
-
 ?
3.2.1.20 additional information transglucosylation activity by wild-type and Asn694 mutant enzymes forming isomaltooligosaccharides, overview. Isomaltose and panose are the predominant final products of wild-type alpha-glucosidase Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 More alpha-glucosidases structure comparisons, overview Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 AGL
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 37
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4
-
 wild-type enzyme Aspergillus niger
3.2.1.20 4.7
-
 mutant N694F Aspergillus niger
3.2.1.20 4.8
-
 mutant N694L Aspergillus niger

General Information

EC Number General Information Comment Organism
3.2.1.20 evolution the enzyme belongs to the glycoside hydrolase family 31, GH31 Aspergillus niger