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Literature summary extracted from

  • Horiuchi, A.; Aslam, M.; Kanai, T.; Atomi, H.
    A structurally novel chitinase from the chitin-degrading hyperthermophilic archaeon Thermococcus chitonophagus (2016), Appl. Environ. Microbiol., 82, 3554-3562 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.201 expression of variants in Escherichia coli, one without the signal sequence and the other corresponding only to the putative catalytic domain Thermococcus chitonophagus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.201 Thermococcus chitonophagus A0A161KIT4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.201 colloidal chitin + H2O
-
 Thermococcus chitonophagus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.201 additional information enzyme hydrolyzes GlcNAc oligomers longer than (GlcNAc)4. ChiD is an exo-type chitinase that releases (GlcNAc)2 or (GlcNAc)3. ChiD recognizes the reducing end of chitin chains Thermococcus chitonophagus ?
-
 ?
3.2.1.201 N, N', N'', N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Thermococcus chitonophagus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.201 N, N', N'', N''',N''''-pentaacetylchitopentaose + H2O
-
 Thermococcus chitonophagus N,N'-diactelchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.201 N, N', N'', N'''-tetraacetylchitotetraose + H2O
-
 Thermococcus chitonophagus 2 N,N'-diacetylchitobiose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.201 ChiD
-
 Thermococcus chitonophagus

General Information

EC Number General Information Comment Organism
3.2.1.201 metabolism the presence of the two chitin-binding domains significantly enhance the termostability of the catalytic domain Thermococcus chitonophagus