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Literature summary extracted from

  • Li, X.; Guo, J.; Hu, Y.; Yang, Y.; Jiang, J.; Nan, F.; Wu, S.; Xin, Z.
    Identification of a novel feruloyl esterase by functional screening of a soil metagenomic library (2018), Appl. Biochem. Biotechnol., 187, 424-437 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 degradation presence of enzyme enhances the quantity of ferulic acid from destarched wheat bran in presence of xylanase uncultured microorganism

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Escherichia coli uncultured microorganism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.015
-
 methyl ferulate pH 5.0, 30°C uncultured microorganism
3.1.1.73 0.035
-
 methyl sinapate pH 5.0, 30°C uncultured microorganism
3.1.1.73 0.067
-
 methyl p-coumarate pH 5.0, 30°C uncultured microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 uncultured microorganism
-
 cosmid metagenomic library from a soil sample
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 40
-
 30°C, pH 5.0 uncultured microorganism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl ferulate + H2O
-
 uncultured microorganism methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 uncultured microorganism methanol + p-coumarate
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 uncultured microorganism methanol + sinapate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 29000, SDS-PAGE, x * 237000, calculated from sequence uncultured microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 30
-
-
 uncultured microorganism

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 6.1
-
 methyl p-coumarate pH 5.0, 30°C uncultured microorganism
3.1.1.73 18.8
-
 methyl sinapate pH 5.0, 30°C uncultured microorganism
3.1.1.73 19.6
-
 methyl ferulate pH 5.0, 30°C uncultured microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 uncultured microorganism

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 uncultured microorganism calculated from sequence
-
 10

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 91
-
 methyl p-coumarate pH 5.0, 30°C uncultured microorganism
3.1.1.73 537
-
 methyl sinapate pH 5.0, 30°C uncultured microorganism
3.1.1.73 1306
-
 methyl ferulate pH 5.0, 30°C uncultured microorganism