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Literature summary extracted from

  • Zhou, Z.; Zhao, S.; Liu, Y.; Chang, Z.; Ma, Y.; Li, J.; Song, J.
    A highly conserved aspartic acid residue of the chitosanase from Bacillus sp. TS is involved in the substrate binding (2016), Appl. Biochem. Biotechnol., 180, 1167-1179 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expression in Escherichia coli BL21 (DE3) Bacillus sp. TS

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 D318A mutant enzyme retains 29.6% of wild-type activity Bacillus sp. TS
3.2.1.132 D318E mutant enzyme retains 16.4% of wild-type activity Bacillus sp. TS
3.2.1.132 D318K mutant enzyme retains 2.95% of wild-type activity Bacillus sp. TS
3.2.1.132 D318N mutant enzyme shows enhanced specific activity relative to wild-type chitosanase Bacillus sp. TS
3.2.1.132 D318R mutant enzyme retains 4.68% of wild-type activity Bacillus sp. TS

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 chitosan Km-value: 1.19 mg/ml, wild-type enzyme, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 1.579 g/ml, mutant enzyme D318N, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 12.33 mg/ml, mutant enzyme D318E, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 12.67 mg/ml, mutant enzyme D318K, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 18.5 mg/ml, mutant enzyme D318A, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 5 mg/ml, mutant enzyme D318R, pH 5.0, 50°C Bacillus sp. TS

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. TS A0A167KLN5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Bacillus sp. TS

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Bacillus sp. TS ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 CsnTS
-
 Bacillus sp. TS

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 57.5
-
 Tm-value of mutant enzymes D318R Bacillus sp. TS
3.2.1.132 57.7
-
 Tm-value of mutant enzymes D318K Bacillus sp. TS
3.2.1.132 63.6
-
 Tm-value of mutant enzyme D318A Bacillus sp. TS
3.2.1.132 67.1
-
 Tm-value of wild-type enzyme Bacillus sp. TS
3.2.1.132 67.5
-
 Tm-value of mutant enzyme D318N Bacillus sp. TS

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 19400
-
 chitosan pH 5.0, 50°C, mutant enzyme D318R Bacillus sp. TS
3.2.1.132 20500
-
 chitosan pH 5.0, 50°C, mutant enzyme D318E Bacillus sp. TS
3.2.1.132 21700
-
 chitosan pH 5.0, 50°C, mutant enzyme D318K Bacillus sp. TS
3.2.1.132 201000
-
 chitosan pH 5.0, 50°C, mutant enzyme D318A Bacillus sp. TS
3.2.1.132 505000
-
 chitosan pH 5.0, 50°C, wild-type enzyme Bacillus sp. TS
3.2.1.132 943000
-
 chitosan pH 5.0, 50°C, mutant enzyme D318N Bacillus sp. TS

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5
-
 optimum pH for wild-type enzyme and mutant enzymes D318R and D318K Bacillus sp. TS

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 chitosan Km-value: 10800 mg/s*ml, mutant enzyme D318A, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 1630 mg/s*ml, mutant enzyme D318E, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 1710 mg/s*ml, mutant enzyme D318K, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 3880 mg/s*ml, mutant enzyme D318R, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 424000 mg/s*ml, wild-type enzyme, pH 5.0, 50°C Bacillus sp. TS
3.2.1.132 additional information
-
 chitosan Km-value: 599000 mg/s*ml, mutant enzyme D318N, pH 5.0, 50°C Bacillus sp. TS