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Literature summary extracted from

  • Yang, D.; Tian, G.; Du, F.; Zhao, Y.; Zhao, L.; Wang, H.; Ng, T.B.
    A fungal alpha-galactosidase from Pseudobalsamia microspora capable of degrading raffinose family oligosaccharides (2015), Appl. Biochem. Biotechnol., 176, 2157-2169 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 carbodiimide 165% activity at 0.4 mM Diehliomyces microsporus
3.2.1.22 diacetyl 140% activity at 0.2 mM Diehliomyces microsporus
3.2.1.22 diethyl dicarbonate 170% activity at 0.4 mM Diehliomyces microsporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Al3+ 46% residual activity at 2.5 mM Diehliomyces microsporus
3.2.1.22 Ca2+ 47% residual activity at 2.5 mM Diehliomyces microsporus
3.2.1.22 Cd2+ complete inhibition at 1.25 mM Diehliomyces microsporus
3.2.1.22 Cu2+ complete inhibition at 1.25 mM Diehliomyces microsporus
3.2.1.22 dithiothreitol 54% residual activity at 0.4 mM Diehliomyces microsporus
3.2.1.22 Fe2+ 44% residual activity at 1.25 mM Diehliomyces microsporus
3.2.1.22 Fe3+ 6% residual activity at 10 mM Diehliomyces microsporus
3.2.1.22 Hg2+ complete inhibition at 1.25 mM Diehliomyces microsporus
3.2.1.22 K+ 32% residual activity at 2.5 mM Diehliomyces microsporus
3.2.1.22 Mg2+ 63% residual activity at 1.25 mM Diehliomyces microsporus
3.2.1.22 Mn2+ 33% residual activity at 5 mM Diehliomyces microsporus
3.2.1.22 N-bromosuccinimide complete inhibition at 0.2 mM Diehliomyces microsporus
3.2.1.22 Pb2+ 29% residual activity at 5 mM Diehliomyces microsporus
3.2.1.22 Zn2+ 24% residual activity at 5 mM Diehliomyces microsporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.29
-
 4-nitrophenyl alpha-D-glucopyranoside at pH 4.6 and 50°C Diehliomyces microsporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 62000
-
 gel filtration Diehliomyces microsporus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Diehliomyces microsporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 DEAE-cellulose column chromatography, Q Sepharose column chromatography, Mono Q column chromatography, and Superdex G-75 gel filtration Diehliomyces microsporus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 9.21
-
 crude extract, at pH 4.6 and 50°C Diehliomyces microsporus
3.2.1.22 11275
-
 after 1224.1fold purification, at pH 4.6 and 50°C Diehliomyces microsporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl alpha-D-glucopyranoside + H2O 100% activity Diehliomyces microsporus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.22 melibiose + H2O 15.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside Diehliomyces microsporus D-glucose + D-galactose
-
 ?
3.2.1.22 additional information no activity with 2-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucuronide, guar gum, and locust bean gum Diehliomyces microsporus ?
-
 ?
3.2.1.22 raffinose + H2O 49.95% activity compared to 4-nitrophenyl alpha-D-glucopyranoside Diehliomyces microsporus sucrose + D-galactose
-
 ?
3.2.1.22 stachyose + H2O 6.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside Diehliomyces microsporus 2 D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 monomer 1 * 62000, SDS-PAGE Diehliomyces microsporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 55
-
-
 Diehliomyces microsporus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.22 30 60 about 40% activity at 30°C, about 55% activity at 40°C, about 95% activity at 50°C, 100% activity at 55°C, about 80% activity at 60°C, almost no activity at 70°C Diehliomyces microsporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
-
 Diehliomyces microsporus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 4 6 the enzyme exhibits 60% and 73% of the maximum activity at pH 4.0 and 6.0, respectively. At pH 3.0 and 7.0, there is hardly any alpha-galactosidase activity remaining Diehliomyces microsporus