Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Su, Q.; Jin, T.; Yu, Y.; Yang, M.; Mou, H.; Li, L.
    Extracellular expression of a novel beta-agarase from Microbulbifer sp. Q7, isolated from the gut of sea cucumber (2017), AMB Express, 7, 220 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B1 2-mercaptoethanol 141.05% activity at 0.5% (v/v) Microbulbifer sp. Q7
3.2.1.B1 dithiothreitol 103.44% activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 additional information not influenced by Triton X-100 Microbulbifer sp. Q7

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B1 expressed in Escherichia coli BL21(DE3) cells Microbulbifer sp. Q7

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B1 Ca2+ 96.78% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 Cu2+ 6.2% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 EDTA 86.47% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 Fe3+ 34.65% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 K+ 97.26% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 Li+ 92% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 Mg2+ 76.92% residual activity at 5 mM Microbulbifer sp. Q7
3.2.1.B1 additional information not inhibited by urea and SDS Microbulbifer sp. Q7
3.2.1.B1 Tween-80 89.72% residual activity at 0.5% (v/v) Microbulbifer sp. Q7
3.2.1.B1 Zn2+ 14.97% residual activity at 5 mM Microbulbifer sp. Q7

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B1 extracellular
-
 Microbulbifer sp. Q7
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B1 Na+ 116.46% activity at 5 mM Microbulbifer sp. Q7

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.B1 agarose + H2O Microbulbifer sp. Q7
-
 neoagarohexaose + neoagarotetraose + D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B1 Microbulbifer sp. Q7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B1 Ni Sepharose column chromatography Microbulbifer sp. Q7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B1 agarose + H2O
-
 Microbulbifer sp. Q7 neoagarohexaose + neoagarotetraose + D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B1 ? x * 65000, SDS-PAGE Microbulbifer sp. Q7

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B1 AgaB
-
 Microbulbifer sp. Q7
3.2.1.B1 beta-agarase
-
 Microbulbifer sp. Q7

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B1 40
-
-
 Microbulbifer sp. Q7

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B1 35 50 more than 50% activity between 35 and 50°C Microbulbifer sp. Q7

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B1 37 45 the enzyme retains 90.12% activity at 37°C after 2 h, more than 55% activity at 40 and 42°C after 1 h, and 36.16% activity at 45°C after 1 h Microbulbifer sp. Q7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B1 6
-
-
 Microbulbifer sp. Q7

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B1 4 10 more than 70% activity between pH 4.0 and 10.0 Microbulbifer sp. Q7

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B1 6 9 the enzyme is stable between pH 6.0 and pH 9.0 for 1 h Microbulbifer sp. Q7