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Literature summary extracted from

  • Bergonzi, C.; Schwab, M.; Chabriere, E.; Elias, M.
    The quorum-quenching lactonase from Alicyclobacter acidoterrestris purification, kinetic characterization, crystallization and crystallographic analysis (2017), Acta Crystallogr. Sect. F, 73, 476-480 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expression in Escherichia coli Alicyclobacillus acidoterrestris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.81 sitting-drop vapor diffusion, X-ray diffraction data collected at 1.65 A resolution Alicyclobacillus acidoterrestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.81 0.0105
-
 N-butanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 0.014
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 0.0496
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 0.0827
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 32500
-
 SDS-PAGE Alicyclobacillus acidoterrestris

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Alicyclobacillus acidoterrestris T0BMH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81
-
 Alicyclobacillus acidoterrestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-3-oxododecanoyl-L-homoserine
-
 ?
3.1.1.81 N-butanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-butanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
 Alicyclobacillus acidoterrestris N-hexanoyl-L-homoserine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.81 monomer 1 * 32500, SDS-PAGE Alicyclobacillus acidoterrestris

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AaL
-
 Alicyclobacillus acidoterrestris
3.1.1.81 quorum-quenching lactonase
-
 Alicyclobacillus acidoterrestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.81 58
-
 Tm-value Alicyclobacillus acidoterrestris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.81 5.03
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 5.13
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 13.54
-
 N-butanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 13.97
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.81 0.103
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 0.169
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 0.36
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris
3.1.1.81 1.29
-
 N-butanoyl-L-homoserine lactone pH 8.3, 25°C Alicyclobacillus acidoterrestris