EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.2.14 | expressed in Escherichia coli BL21(DE3) cells | Umbellularia californica |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.2.14 | hanging drop vapor diffusion method, using 0.2 M (NH4)2SO4, 0.1 M Tris-HCl pH 8.5, 20% (w/v) PEG3350 | Umbellularia californica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.2.14 | F201W | the mutant shows 14.1% specific activity compared to the wild type enzyme | Umbellularia californica |
3.1.2.14 | M163W | almost inactive | Umbellularia californica |
3.1.2.14 | M197R | the mutant shows 39.8% specific activity compared to the wild type enzyme | Umbellularia californica |
3.1.2.14 | N283A | almost inactive | Umbellularia californica |
3.1.2.14 | R199H | the mutant shows 0.4% specific activity compared to the wild type enzyme | Umbellularia californica |
3.1.2.14 | S219W | almost inactive | Umbellularia californica |
3.1.2.14 | T137G | the mutant demonstrates comparative relative activity on C14 substrates compared to C12 substrates in vitro and achieves C14 fatty acid content up to 40% in contrast to 10% C14 from the wild type | Umbellularia californica |
3.1.2.14 | T137Y | inactive | Umbellularia californica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.2.14 | chloroplast | - |
Umbellularia californica | 9507 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.14 | lauroyl-[acyl-carrier protein] + H2O | Umbellularia californica | - |
[acyl-carrier protein] + laurate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.14 | Umbellularia californica | Q41635 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.14 | Ni-NTA column chromatography | Umbellularia californica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.14 | caproyl-[acyl-carrier protein] + H2O | very low activity | Umbellularia californica | [acyl-carrier protein] + caproate | - |
? | |
3.1.2.14 | lauroyl-[acyl-carrier protein] + H2O | - |
Umbellularia californica | [acyl-carrier protein] + laurate | - |
? | |
3.1.2.14 | lauroyl-[acyl-carrier protein] + H2O | high activity | Umbellularia californica | [acyl-carrier protein] + laurate | - |
? | |
3.1.2.14 | myristoyl-[acyl-carrier protein] + H2O | very low activity | Umbellularia californica | [acyl-carrier protein] + myristate | - |
? | |
3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | very low activity | Umbellularia californica | [acyl-carrier protein] + palmitate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.2.14 | homodimer | x-ray crystallography | Umbellularia californica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.14 | acyl-ACP-thioesterase | - |
Umbellularia californica |
3.1.2.14 | C12-specific thioesterase | - |
Umbellularia californica |
3.1.2.14 | FatB | - |
Umbellularia californica |