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Literature summary extracted from

  • Thapa, H.R.; Tang, S.; Sacchettini, J.C.; Devarenne, T.P.
    Tetraterpene Synthase Substrate and Product Specificity in the Green Microalga Botryococcus braunii Race L (2017), ACS Chem. Biol., 12, 2408-2416 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.148 A288F significant loss of lycopaoctaene production when compared to wild-type. Increased residue size in the mutant blocks binding of geranylgeranyl diphosphate in the S1 pocket Botryococcus braunii
2.5.1.148 M180L mutant retains the ability to produce lycopaoctaene Botryococcus braunii
2.5.1.148 additional information mutating Ser276 and Ala288 residues to those found in human squalene synthase largely converts LOS from lycopaoctaene production to C30 squalene production Botryococcus braunii
2.5.1.148 S276Y significant loss of lycopaoctaene production when compared to wild-type. Increased residue size in the mutant blocks binding of geranylgeranyl diphosphate in the S1 pocket Botryococcus braunii
2.5.1.148 S276Y/A288F mutant shows a drastic reduction in the ability to produce lycopaoctaene and C35H58 compared to wild-type enzyme Botryococcus braunii
2.5.1.148 T65V mutant retains the ability to produce lycopaoctaene Botryococcus braunii
2.5.1.148 T65V/A288F when comparing the M7 double mutant to the parent A288F background, T65V/A288F does not show a difference in lycopaoctaene production, but C35H58 production is slightly increased and squalene formation is significantly reduced Botryococcus braunii
2.5.1.148 T65V/M180L activity of the mutant enzyme is similar to that of the wild-type enzyme Botryococcus braunii
2.5.1.148 T65V/M180L/A288F mutant shows activity similar to that of the M4 parent background Botryococcus braunii
2.5.1.148 T65V/M180L/S276Y A288F in comparison to the M9 parent background, the mutant enzyme shows a further reduction in C35H58 production and no significant change in lycopaoctaene and squalene formation Botryococcus braunii
2.5.1.148 T65V/M180L/S276Y/A288F/V289C in comparison to the M9 parent background, the mutant enzyme shows a further reduction in C35H58 production and no significant change in lycopaoctaene and squalene formation Botryococcus braunii
2.5.1.148 V289C mutant retains the ability to produce lycopaoctaene Botryococcus braunii
2.5.1.148 V289C/S276Y for the V289C/S276Y double mutant, in comparison to the S276Y parent background, a small increase in C35H58 production is observed, whereas the activities for lycopaoctaene and squalene production remain the same Botryococcus braunii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.148 2 geranylgeranyl diphosphate + NADPH + H+ Botryococcus braunii the enzyme is involved in biosynthesis of (14E,18E)-lycopadiene overall reaction. Lycopaoctaene i.e. 15,15'-dihydrophytoene = (6E,10E,14E,18E,22E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaene lycopaoctaene + 2 diphosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.148 Botryococcus braunii A0A142ZC57
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.148 2 farnesyl diphosphate + NADPH + H+
-
 Botryococcus braunii C30 squalene + 2 diphosphate + NADP+
-
 ?
2.5.1.148 2 geranylgeranyl diphosphate + NADPH + H+ the enzyme is involved in biosynthesis of (14E,18E)-lycopadiene overall reaction. Lycopaoctaene i.e. 15,15'-dihydrophytoene = (6E,10E,14E,18E,22E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaene Botryococcus braunii lycopaoctaene + 2 diphosphate + NADP+
-
 ?
2.5.1.148 2 geranylgeranyl diphosphate + NADPH + H+ overall reaction. Lycopaoctaene i.e. 15,15'-dihydrophytoene = (6E,10E,14E,18E,22E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaene. The enzyme catalyzes the head-to-head condensation of two C20 geranylgeranyl diphosphate molecules to produce C40 lycopaoctaene Botryococcus braunii lycopaoctaene + 2 diphosphate + NADP+
-
 ?
2.5.1.148 geranylgeranyl diphosphate + farnesyl diphosphate + NADPH + H+
-
 Botryococcus braunii C35H58 hydrocarbon + 2 diphosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.148 LOS
-
 Botryococcus braunii

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.148 NADPH
-
 Botryococcus braunii

General Information

EC Number General Information Comment Organism
2.5.1.148 metabolism the enzyme is involved in biosynthesis of (14E,18E)-lycopadiene. Lycopaoctaene i.e. 15,15'-dihydrophytoene = (6E,10E,14E,18E,22E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaene Botryococcus braunii