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Literature summary extracted from

  • Ben Bdira, F.; Kallemeijn, W.W.; Oussoren, S.V.; Scheij, S.; Bleijlevens, B.; Florea, B.I.; van Roomen, C.P.A.A.; Ottenhoff, R.; van Kooten, M.J.F.M.; Walvoort, M.T.C.; Witte, M.D.; Boot, R.G.; Ubbink, M.; Overkleeft, H.S.; Aerts, J.M.F.G.
    Stabilization of glucocerebrosidase by active site occupancy (2017), ACS Chem. Biol., 12, 1830-1841 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.45 L444P the mutant enzyme exhibits impaired endoplasmic reticulum folding Homo sapiens
3.2.1.45 N370S the mutant enzyme is labile in lysosomes Homo sapiens

General Stability

EC Number General Stability Organism
3.2.1.45 cyclophellitol-derived activity-based probes improve the stability of the enzyme in vitro. At pH 5.2, the recombinant enzyme shows resistance to tryptic digestion over the course of 90 min, whereas at pH 7.4, the enzyme is more sensitive to trypsin digestion, with about 40% degraded within 60 min Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.45 conduritol beta-epoxide irreversible inhibitor Homo sapiens
3.2.1.45 cyclophellitol beta-epoxide ABP4
-
 Homo sapiens
3.2.1.45 cyclophellitol beta-epoxide ABP5
-
 Homo sapiens
3.2.1.45 isofagomine potent, reversible inhibitor Homo sapiens
3.2.1.45 triazole-linked 6-BODIPY-2-deoxy-2-fluoro-beta-D-glucopyranosyl-N-phenyltrifluoroacetimidate inhibitor semi-irreversible inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.45 lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.45 glucosylceramide + H2O Homo sapiens
-
 D-glucose + ceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.45 Homo sapiens P04062
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.45 concanavalin A-Sepharose column chromatography and Superdex 75 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.45 fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.45 4-methylumbelliferyl beta-D-glucoside
-
 Homo sapiens 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.45 glucosylceramide + H2O
-
 Homo sapiens D-glucose + ceramide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.45 ? x * 58000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.45 GBA
-
 Homo sapiens
3.2.1.45 glucocerebrosidase
-
 Homo sapiens
3.2.1.45 Imiglucerase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.45 57
-
 melting temperature of the recombinant enzyme at pH 7.4 Homo sapiens
3.2.1.45 61
-
 melting temperature of the recombinant enzyme at pH 5.2 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.45 5.2 7.4 the activity of the recombinant enzyme is preserved under acidic conditions, whereas at pH 7.4, its activity is lost with a half-life of 30 min Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.45 0.0000058
-
 at pH 7.0 and 37°C Homo sapiens isofagomine
3.2.1.45 0.0000314
-
 at pH 5.2 and 37°C Homo sapiens isofagomine
3.2.1.45 0.005458
-
 at pH 5.2 and 37°C Homo sapiens triazole-linked 6-BODIPY-2-deoxy-2-fluoro-beta-D-glucopyranosyl-N-phenyltrifluoroacetimidate inhibitor
3.2.1.45 0.009497
-
 at pH 5.2 and 37°C Homo sapiens conduritol beta-epoxide

General Information

EC Number General Information Comment Organism
3.2.1.45 malfunction enzyme deficiency results in Gaucher disease Homo sapiens