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Literature summary extracted from

  • Hossain, M.S.; Tanaka, T.; Takagi, K.; Hayashi, J.; Wakayama, M.
    Purification and characterization of Stenotrophomonas maltophilia-derived l-amino acid ester hydrolase for synthesizing dipeptide, isoleucyl-tryptophan (2018), 3 Biotech, 8, 173 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.43 Ag+ 1 mM, complete inhibition Stenotrophomonas maltophilia
3.1.1.43 Ca2+ 1 mM, 25% inhibition Stenotrophomonas maltophilia
3.1.1.43 Cu2+ 1 mM, 25% inhibition Stenotrophomonas maltophilia
3.1.1.43 Fe2+ 1 mM, 30% inhibition Stenotrophomonas maltophilia
3.1.1.43 Li+ 1 mM, 30% inhibition Stenotrophomonas maltophilia
3.1.1.43 phenylmethanesulfonyl fluoride 1 mM, complete inhibition Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.43 Na+ 1 mM, 130% of initial activity Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.43 300000
-
 gel filtration Stenotrophomonas maltophilia

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.43 Stenotrophomonas maltophilia A0A2Z6IAJ2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.43 5.34
-
 substrate L-isoleucyl methyl ester, pH 7.0, 30°C Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.43 beta-alanyl methyl ester + L-tryptophan about 100% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia beta-alanyl-L-tryptophan + methanol
-
 ?
3.1.1.43 glycyl methyl ester + L-tryptophan about 230% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia glycyl-L-tryptophan + methanol
-
 ?
3.1.1.43 L-alanyl methyl ester + L-tryptophan about 240% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-alanyl-L-tryptophan + methanol
-
 ?
3.1.1.43 L-arginyl methyl ester + L-tryptophan about 220% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-arginyl-L-tryptophan + methanol
-
 ?
3.1.1.43 L-aspartyl methyl ester + L-tryptophan about 200% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-aspartyl-L-tryptophan + methanol
-
 ?
3.1.1.43 L-isoleucyl methyl ester + L-tryptophan
-
 Stenotrophomonas maltophilia L-isoleucyl-L-tryptophan + methanol
-
 ?
3.1.1.43 L-proyl methyl ester + L-tryptophan about 240% of the activity with L-isoleucyl methyl ester Stenotrophomonas maltophilia L-prolyl-L-tryptophan + methanol
-
 ?
3.1.1.43 L-tryptophanyl methyl ester + L-tryptophan
-
 Stenotrophomonas maltophilia L-tryptophanyl-L-tryptophan + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.43 homotetramer 4 * 70000, SDS-PAGE Stenotrophomonas maltophilia

Synonyms

EC Number Synonyms Comment Organism
3.1.1.43 AEH
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.43 30
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.43 50
-
 10 min, 40% loss of activity Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.43 9
-
-
 Stenotrophomonas maltophilia