EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Triton X-100 | isoform Ag-I shows 110% activity at 1% (v/v) Triton X-100 | Acinetobacter sp. | |
3.2.1.22 | Tween 20 | isoform Ag-I shows 128% activity and isoform at 1% (v/v) Tween 20 | Acinetobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | 2-mercaptoethanol | isoform Ag-I shows 88% residual activity and isoform Ag-II shows 74% residual activity at 5 mM 2-mercaptoethanol | Acinetobacter sp. | |
3.2.1.22 | Ag+ | isoform Ag-I shows 7% residual activity and isoform Ag-II is completely inhibited by 5 mM Ag+ | Acinetobacter sp. | |
3.2.1.22 | Ca2+ | isoform Ag-I shows 90% residual activity and isoform Ag-II shows 67% residual activity at 5 mM Ca2+ | Acinetobacter sp. | |
3.2.1.22 | Cu2+ | isoform Ag-I shows 80% residual activity and isoform Ag-II shows 56% residual activity at 5 mM Cu2+ | Acinetobacter sp. | |
3.2.1.22 | D-galactose | isoform Ag-I shows 92% residual activity and isoform Ag-II shows 72% residual activity at 5 mM D-galactose | Acinetobacter sp. | |
3.2.1.22 | D-glucose | isoform Ag-I shows 82% residual activity and isoform Ag-II shows 75% residual activity at 5 mM D-glucose | Acinetobacter sp. | |
3.2.1.22 | Hg2+ | isoform Ag-I shows 5% residual activity and isoform Ag-II is completely inhibited by 5 mM Hg2+ | Acinetobacter sp. | |
3.2.1.22 | K+ | isoform Ag-I shows 98% residual activity and isoform Ag-II shows 67% residual activity at 5 mM K+ | Acinetobacter sp. | |
3.2.1.22 | lactose | isoform Ag-I shows 74% residual activity and isoform Ag-II shows 70% residual activity at 5 mM lactose | Acinetobacter sp. | |
3.2.1.22 | additional information | not inhibited by EDTA | Acinetobacter sp. | |
3.2.1.22 | p-chloromercuribenzoate | complete inhibition at 5 mM | Acinetobacter sp. | |
3.2.1.22 | SDS | complete inhibition at 1% (w/v) | Acinetobacter sp. | |
3.2.1.22 | stachyose | isoform Ag-I shows 71% residual activity and isoform Ag-II shows 63% residual activity at 5 mM D-stachyose | Acinetobacter sp. | |
3.2.1.22 | Urea | isoform Ag-II shows 92% residual activity at 5 mM urea | Acinetobacter sp. | |
3.2.1.22 | Zn2+ | isoform Ag-I shows 90% residual activity and isoform Ag-II shows 75% residual activity at 5 mM Zn2+ | Acinetobacter sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Co2+ | isoform Ag-I shows 105% activity at 5 mM Mg2+ | Acinetobacter sp. | |
3.2.1.22 | Mg2+ | isoform Ag-I shows 108% activity at 5 mM Mg2+ | Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | raffinose + H2O | Acinetobacter sp. | - |
sucrose + D-galactose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Acinetobacter sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration | Acinetobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 1.7 | - |
crude extract, at pH 7.0 and 36°C | Acinetobacter sp. |
3.2.1.22 | 8 | - |
isoform Ag-II, after 4.7fold purification, at pH 7.0 and 36°C | Acinetobacter sp. |
3.2.1.22 | 12 | - |
isoform Ag-I, after 7fold purification, at pH 7.0 and 36°C | Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Acinetobacter sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Acinetobacter sp. | sucrose + D-galactose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.22 | monomer | 1 * 37000, isoform Ag-II, SDS-PAGE | Acinetobacter sp. |
3.2.1.22 | monomer | 1 * 65000, isoform Ag-I, SDS-PAGE | Acinetobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | Ag-I | isoform | Acinetobacter sp. |
3.2.1.22 | Ag-II | isoform | Acinetobacter sp. |
3.2.1.22 | alpha-galactosidase I | isoform | Acinetobacter sp. |
3.2.1.22 | alpha-galactosidase II | isoform | Acinetobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 40 | - |
isoform Ag-II | Acinetobacter sp. |
3.2.1.22 | 50 | 60 | isoform Ag-I | Acinetobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 70 | 90 | the half-life of isoform Ag-I varies from 1.85 h at 90°C to 7.6 h at 70°C | Acinetobacter sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 1 | - |
raffinose | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.2.1.22 | 1.83 | - |
raffinose | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.2.1.22 | 2.53 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.2.1.22 | 7.42 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 7 | 9 | isoform Ag-I is highly active and stable over broad pH range showing more than 60% activity up to pH 9.0 with low activities at acidic pH. After 24 h of incubation at 36°C, isoform Ag-I retains 85% enzyme activity at pH 7.0, whereas isoform Ag-II retains 52% activity | Acinetobacter sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.55 | - |
raffinose | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.2.1.22 | 1.35 | - |
raffinose | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.2.1.22 | 3.72 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.2.1.22 | 22.47 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. |