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Literature summary extracted from

  • Ji, W.; Sun, W.; Feng, J.; Song, T.; Zhang, D.; Ouyang, P.; Gu, Z.; Xie, J.
    Characterization of a novel N-acetylneuraminic acid lyase favoring industrial N-acetylneuraminic acid synthesis (2015), Sci. Rep., 5, 9341 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.3 EDTA about 120% activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis and about 150% activity at 5 mM and pH 8.5 for N-acetylneuraminate cleavage Corynebacterium glutamicum
4.1.3.3 Triton X-100 about 150% activity at 5 mM and pH 8.5 for N-acetylneuraminate synthesis and about 220% activity at 5 mM and pH 8.5 for N-acetylneuraminate cleavage Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.3 expressed in Escherichia coli Rosetta (DE3) cells Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 CTAB about 30% residual activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis and almost complete inhibition at 5 mM for N-acetylneuraminate cleavage Corynebacterium glutamicum
4.1.3.3 SDS about 30% residual activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis and almost complete inhibition at 5 mM for N-acetylneuraminate cleavage Corynebacterium glutamicum
4.1.3.3 Zn2+ about 25% residual activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis and almost complete inhibition at 5 mM and pH 7.5 for N-acetylneuraminate cleavage Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 3 3.5 N-acetylneuraminate at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 14.7
-
 pyruvate at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 53.3
-
 N-acetyl-D-mannosamine at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 72.4
-
 pyruvate at pH 8.5 and 37°C Corynebacterium glutamicum
4.1.3.3 87.7
-
 N-acetylneuraminate at pH 8.5 and 37°C Corynebacterium glutamicum
4.1.3.3 92.1
-
 N-acetyl-D-mannosamine at pH 8.5 and 37°C Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.3 Ba2+ about 170% activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis Corynebacterium glutamicum
4.1.3.3 Co2+ about 225% activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis Corynebacterium glutamicum
4.1.3.3 Fe3+ about 125% activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis Corynebacterium glutamicum
4.1.3.3 Mg2+ about 270% activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis Corynebacterium glutamicum
4.1.3.3 Ni2+ about 220% activity at 5 mM and pH 7.5 for N-acetylneuraminate synthesis Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.3 N-acetylneuraminate Corynebacterium glutamicum
-
 N-acetyl-D-mannosamine + pyruvate
-
 r
4.1.3.3 N-acetylneuraminate Corynebacterium glutamicum ATCC 13032
-
 N-acetyl-D-mannosamine + pyruvate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Corynebacterium glutamicum Q8NMD2
-
-
4.1.3.3 Corynebacterium glutamicum ATCC 13032 Q8NMD2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.3 Ni-NTA agarose column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-acetyl-D-mannosamine + pyruvate
-
 Corynebacterium glutamicum N-acetylneuraminate
-
 r
4.1.3.3 N-acetyl-D-mannosamine + pyruvate
-
 Corynebacterium glutamicum ATCC 13032 N-acetylneuraminate
-
 r
4.1.3.3 N-acetylneuraminate
-
 Corynebacterium glutamicum N-acetyl-D-mannosamine + pyruvate
-
 r
4.1.3.3 N-acetylneuraminate
-
 Corynebacterium glutamicum ATCC 13032 N-acetyl-D-mannosamine + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.3 ? x * 33000, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
4.1.3.3 N-Acetylneuraminic acid lyase
-
 Corynebacterium glutamicum
4.1.3.3 NAL
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.3 40
-
 for N-acetylneuraminate cleavage at pH 7.5 and N-acetylneuraminate synthesis at pH 8.5 Corynebacterium glutamicum
4.1.3.3 40 50 for N-acetylneuraminate synthesis at pH 7.5 Corynebacterium glutamicum
4.1.3.3 45
-
 for N-acetylneuraminate cleavage at pH 8.5 Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.3 40
-
 when the enzyme is incubated at 40°C, pH 8.5 for 48 h, in N-acetylneuraminate synthesis direction, the enzyme keeps 100% activity for the first 24 h but remains 60% of its activity in 48h. 75% and 20% of N-acetylneuraminate cleavage activity remain in 24 h and 48 h, respectively Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.3 5.67
-
 N-acetyl-D-mannosamine at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 6.1
-
 pyruvate at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 9.3
-
 N-acetylneuraminate at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 40.67
-
 N-acetyl-D-mannosamine at pH 8.5 and 37°C Corynebacterium glutamicum
4.1.3.3 42.58
-
 pyruvate at pH 8.5 and 37°C Corynebacterium glutamicum
4.1.3.3 44.2
-
 N-acetylneuraminate at pH 8.5 and 37°C Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 8.2 8.4 for N-acetylneuraminate cleavage Corynebacterium glutamicum
4.1.3.3 8.6
-
 for N-acetylneuraminate synthesis Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.3.3 0.07
-
 N-acetyl-D-mannosamine at pH 8.5 and 37°C Corynebacterium glutamicum
4.1.3.3 0.11
-
 N-acetyl-D-mannosamine at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 0.11
-
 pyruvate at pH 8.5 and 37°C Corynebacterium glutamicum
4.1.3.3 0.28
-
 N-acetylneuraminate at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 0.41
-
 pyruvate at pH 7.5 and 37°C Corynebacterium glutamicum
4.1.3.3 23.75
-
 N-acetylneuraminate at pH 8.5 and 37°C Corynebacterium glutamicum