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Literature summary extracted from
- Impact of different alginate lyases on combined cellulase-lyase saccharification of brown seaweed (2016), RSC Adv., 6, 45392-45401 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.2.2.3 | degradation | enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata | Sphingomonas sp. A1 |
| 4.2.2.3 | degradation | enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata | Flavobacterium sp. S20 |
| 4.2.2.11 | degradation | enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata | Sphingobacterium multivorum |
| 4.2.2.11 | degradation | enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata | Sphingomonas sp. A1 |
| 4.2.2.11 | degradation | enzymatic treatment for 24 hours is sufficient to release all potential glucose from the glucan rich brown seaweed Laminaria digitata | Flavobacterium sp. S20 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.3 | expression in Escherichia coli | Sphingomonas sp. A1 |
| 4.2.2.3 | expression in Escherichia coli | Flavobacterium sp. S20 |
| 4.2.2.11 | expression in Escherichia coli | Sphingomonas sp. A1 |
| 4.2.2.11 | expression in Escherichia coli | Flavobacterium sp. S20 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.3 | Flavobacterium sp. S20 | G9CHX6 | - |
- |
| 4.2.2.3 | Sphingomonas sp. A1 | Q75WP3 | - |
- |
| 4.2.2.11 | Flavobacterium sp. S20 | G9CHX6 | - |
- |
| 4.2.2.11 | Sphingobacterium multivorum | - |
- |
- |
| 4.2.2.11 | Sphingomonas sp. A1 | Q75WP3 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.2.11 | commercial preparation | - |
Sphingobacterium multivorum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.3 | alginate | - |
Sphingomonas sp. A1 | unsaturated algino-oligosaccharides | - |
? |  |
| 4.2.2.3 | alginate | - |
Flavobacterium sp. S20 | unsaturated algino-oligosaccharides | - |
? | |
| 4.2.2.3 | additional information | enzyme prefers poly-D-mannuronate blocks over poly-L-guluronate blocks | Flavobacterium sp. S20 | ? | - |
? | |
| 4.2.2.3 | additional information | enzyme prefers poly-L-guluronate blocks over poly-D-mannuronate blocks | Sphingomonas sp. A1 | ? | - |
? | |
| 4.2.2.3 | poly(alpha-(1,4)-L-guluronate) | reaction of EC 4.2.2.11 | Sphingomonas sp. A1 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| 4.2.2.3 | poly(alpha-1,4-L-guluronate) | reaction of EC 4.2.2.11 | Flavobacterium sp. S20 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| 4.2.2.3 | poly(beta-(1,4)-D-mannuronate) | - |
Sphingomonas sp. A1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
| 4.2.2.3 | poly(beta-1,4-D-mannuronate) | - |
Flavobacterium sp. S20 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
| 4.2.2.11 | alginate | - |
Sphingobacterium multivorum | unsaturated algino-oligosaccharides | - |
? | |
| 4.2.2.11 | alginate | - |
Sphingomonas sp. A1 | unsaturated algino-oligosaccharides | - |
? | |
| 4.2.2.11 | alginate | - |
Flavobacterium sp. S20 | unsaturated algino-oligosaccharides | - |
? | |
| 4.2.2.11 | additional information | enzyme acts only on poly-guluronate | Sphingobacterium multivorum | ? | - |
? | |
| 4.2.2.11 | additional information | enzyme prefers polyG blocks over polyM blocks | Sphingomonas sp. A1 | ? | - |
? | |
| 4.2.2.11 | additional information | enzyme prefers polyM blocks over polyG blocks | Flavobacterium sp. S20 | ? | - |
? | |
| 4.2.2.11 | poly(alpha-(1,4)-L-guluronate) | - |
Sphingobacterium multivorum | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| 4.2.2.11 | poly(alpha-(1,4)-L-guluronate) | - |
Sphingomonas sp. A1 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| 4.2.2.11 | poly(alpha-(1,4)-L-guluronate) | - |
Flavobacterium sp. S20 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
| 4.2.2.11 | poly(beta-(1,4)-D-mannuronate) | reaction of EC 4.2.2.3 | Sphingomonas sp. A1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
| 4.2.2.11 | poly(beta-(1,4)-D-mannuronate) | reaction of EC 4.2.2.3 | Flavobacterium sp. S20 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.3 | Alg2A | - |
Flavobacterium sp. S20 |
| 4.2.2.11 | Alg2A | - |
Sphingobacterium multivorum |
| 4.2.2.11 | Alg2A | - |
Flavobacterium sp. S20 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.3 | 50 | - |
stable up to | Sphingomonas sp. A1 |
| 4.2.2.11 | 50 | - |
stable up to | Sphingomonas sp. A1 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.3 | 5.5 | 7 | - |
Sphingomonas sp. A1 |
| 4.2.2.3 | 7.5 | - |
- |
Flavobacterium sp. S20 |
| 4.2.2.11 | 5.5 | 7 | - |
Sphingomonas sp. A1 |
| 4.2.2.11 | 7.5 | - |
- |
Sphingobacterium multivorum |
| 4.2.2.11 | 7.5 | - |
- |
Flavobacterium sp. S20 |
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Release 2023.1 (January 2023)
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