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Literature summary extracted from

  • Tran, C.; Nosworthy, N.; Kondyurin, A.; McKenzie, D.; Bilek, M.
    CelB and beta-glucosidase immobilization for carboxymethyl cellulose hydrolysis (2013), RSC Adv., 3, 23604-23611 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.91 biofuel production cellulose hydrolysis is an important step in the production of bioethanol from cellulosic biomass. Two key cellulase enzymes, celB from Caldicellulosiruptor saccharolyticus and beta-glucosidase, are covalently immobilised on polystyrene treated with plasma immersion ion implantation (PIII) which creates radicals that form covalent bonds. The immobilized enzymes are used to produce glucose from carboxymethyl cellulose (CMC), a solubilised form of cellulose. The highest activity of the immobilised celB on PIII treated surfaces was achieved when their immobilisation is carried out at a pH in the range 5-6.5. The immobilized celB on the PIII treated surface had the same activation energy as free celB showing substrate accessibility is not affected by the presence of the surface. The Vmax and Km values of immobilized celB are comparable to those of equal free celB concentrations. The areal density of immobilized celB on the PIII treated surface is estimated to be 0.0003 mg/cm2. The polystyrene surface with immobilized celB at 45°C can be reused over four times (23 hours each) with approximately 30% total activity loss. High ratios of beta-glucosidase to celB enhance the activity of immobilized celB for hydrolysis of carboxymethyl cellulose Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Caldicellulosiruptor saccharolyticus P10474
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 carboxymethyl cellulose + H2O
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 Caldicellulosiruptor saccharolyticus ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CelB
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 Caldicellulosiruptor saccharolyticus

General Information

EC Number General Information Comment Organism
3.2.1.91 additional information celB and beta-glucosidase function effectively together to produce glucose from carboxymethyl cellulose covalently immobilised on plasma immersion ion implantation treated polystyrene. Recombinant celB is optimally immobilised in a pH range 5-6.5 and exhibits a maximum activity when used at pH 5.5. Immobilized celB shows a similar activation energy to the free enzyme in hydrolysis of carboxymethyl cellulose. The areal density of immobilized celB is estimated to be 0.3 mg/cm2. Immobilized celB retains 70% of its initial activity after 4 uses of 23 hours each. High ratio of immobilized beta-glucosidase enhances the activity of immobilized celB in hydrolysis of carboxymethyl cellulose Caldicellulosiruptor saccharolyticus