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Literature summary extracted from

  • Liu, X.; Huang, A.; Luo, D.; Liu, H.; Han, H.; Xu, Y.; Liang, P.
    Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli (2015), Protein Expr. Purif., 109, 79-84 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 an adenylate kinase-T4 DNA ligase fusion protein is expressed in Escherichia coli XL1-Blue cells Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Tequatrovirus T4
-
 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.1 ATP + [DNA ligase]-L-lysine Tequatrovirus T4
-
 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 Blue Sepharose and Ni-NTA column chromatography Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Tequatrovirus T4 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.1 ATP + [DNA ligase]-L-lysine
-
 Tequatrovirus T4 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 T4 DNA ligase
-
 Tequatrovirus T4