EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.271 | expressed in Escherichia coli JM109 cells | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.271 | 0.9 | - |
L-azetidine-2-carboxylic acid | at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
2.3.1.271 | 7.01 | - |
DELTA1-Pyrroline-5-carboxylate | at pH 7.5 and 30°C | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.271 | Saccharomyces cerevisiae | E9P8D2 | - |
- |
2.3.1.271 | Saccharomyces cerevisiae Sigma1278b | E9P8D2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.271 | acetyl-CoA + DELTA1-pyrroline-5-carboxylate + H2O | - |
Saccharomyces cerevisiae | CoA + N-acetyl-glutamate-gamma-semialdehyde | - |
? | |
2.3.1.271 | acetyl-CoA + DELTA1-pyrroline-5-carboxylate + H2O | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-glutamate-gamma-semialdehyde | - |
? | |
2.3.1.271 | acetyl-CoA + L-azetidine-2-carboxylic acid | - |
Saccharomyces cerevisiae | CoA + N-acetyl-L-azetidine-2-carboxylic acid | - |
? | |
2.3.1.271 | acetyl-CoA + L-azetidine-2-carboxylic acid | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-L-azetidine-2-carboxylic acid | - |
? | |
2.3.1.271 | acetyl-CoA + L-glutamate-5-semialdehyde | - |
Saccharomyces cerevisiae | CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? | |
2.3.1.271 | acetyl-CoA + L-glutamate-5-semialdehyde | - |
Saccharomyces cerevisiae Sigma1278b | CoA + N-acetyl-L-glutamate 5-semialdehyde | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.271 | MPR1 | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.271 | 17.9 | - |
L-azetidine-2-carboxylic acid | at pH 8.5 and 30°C | Saccharomyces cerevisiae | |
2.3.1.271 | 121 | - |
DELTA1-Pyrroline-5-carboxylate | at pH 7.5 and 30°C | Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.271 | physiological function | the enzyme protects yeast cells from DELTA1-pyrroline-5-carboxylate-induced oxidative stress by reducing reactive oxygen species | Saccharomyces cerevisiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.271 | 17.2 | - |
DELTA1-Pyrroline-5-carboxylate | at pH 7.5 and 30°C | Saccharomyces cerevisiae | |
2.3.1.271 | 19.8 | - |
L-azetidine-2-carboxylic acid | at pH 8.5 and 30°C | Saccharomyces cerevisiae |