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Literature summary extracted from
- Terpene synthases from Cannabis sativa (2017), PLoS ONE, 12, e0173911 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.B69 | gene CsTPS13PK, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis | Cannabis sativa |
| 4.2.3.106 | gene CsTPS6FN, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis | Cannabis sativa |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.B69 | Mg2+ | required | Cannabis sativa |  |
| 4.2.3.106 | Mg2+ | required | Cannabis sativa | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.B69 | geranyl diphosphate | Cannabis sativa | - |
(Z)-beta-ocimene + diphosphate | - |
? | |
| 4.2.3.106 | geranyl diphosphate | Cannabis sativa | - |
(E)-beta-ocimene + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.B69 | Cannabis sativa | A0A1V0QSG1 | var. Finola | - |
| 4.2.3.106 | Cannabis sativa | A0A1V0QSH1 | var. Finola | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.B69 | additional information | transcriptome analysis of trichomes of the cannabis hemp variety Finola reveals sequences of all stages of terpene biosynthesis. Major terpene compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene. Transcripts associated with terpene biosynthesis are highly expressed in trichomes compared to non-resin producing tissues. Relative composition of terpene profiles in Cannabis sativa var. Finola pistillate flowers, overview | Cannabis sativa | - |
| 4.2.3.B69 | trichome | glandular | Cannabis sativa | - |
| 4.2.3.106 | additional information | transcriptome analysis of trichomes of the cannabis hemp variety Finola reveals sequences of all stages of terpene biosynthesis. Major terpene compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene. Transcripts associated with terpene biosynthesis are highly expressed in trichomes compared to non-resin producing tissues. Relative composition of terpene profiles in Cannabis sativa var. Finola pistillate flowers, overview | Cannabis sativa | - |
| 4.2.3.106 | trichome | glandular | Cannabis sativa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.B69 | geranyl diphosphate | - |
Cannabis sativa | (Z)-beta-ocimene + diphosphate | - |
? | |
| 4.2.3.B69 | geranyl diphosphate | preferred substrate | Cannabis sativa | (Z)-beta-ocimene + diphosphate | - |
? | |
| 4.2.3.B69 | additional information | the enzyme also shows low activity with farnesyl diphosphate. Reaction products are identified by GC-MS analysis | Cannabis sativa | ? | - |
? | |
| 4.2.3.106 | geranyl diphosphate | - |
Cannabis sativa | (E)-beta-ocimene + diphosphate | - |
? | |
| 4.2.3.106 | additional information | the enzyme shows no activity with farnesyl diphosphate. Reaction products are identified by GC-MS analysis | Cannabis sativa | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.B69 | (Z)-beta-ocimene synthase | - |
Cannabis sativa |
| 4.2.3.B69 | CsTPS13PK | - |
Cannabis sativa |
| 4.2.3.106 | CsTPS6FN | - |
Cannabis sativa |
| 4.2.3.106 | CsTPS6PK | - |
Cannabis sativa |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.3.B69 | evolution | the enzyme is a member of the terpene synthase (TPS) superfamily, CsTPSFN TPS-b subfamily. Gene CsTPS13PK, encoding (Z)-beta-ocimene synthase, shares 95.5% amino acid sequence identity with gene CsTPS6FN, encoding (E)-beta-ocimene synthase | Cannabis sativa |
| 4.2.3.B69 | metabolism | transcriptome analysis of trichomes of the cannabis hemp variety Finola reveal sequences of all stages of terpene biosynthesis. Nine cannabis terpene synthases (CsTPS) are identified in subfamilies TPS-a and TPS-b. Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of Finola resin, including major compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene | Cannabis sativa |
| 4.2.3.106 | evolution | the enzyme is a member of the terpene synthase (TPS) superfamily, CsTPSFN TPS-b subfamily | Cannabis sativa |
| 4.2.3.106 | metabolism | transcriptome analysis of trichomes of the cannabis hemp variety Finola reveal sequences of all stages of terpene biosynthesis. Nine cannabis terpene synthases (CsTPS) are identified in subfamilies TPS-a and TPS-b. Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of Finola resin, including major compounds such as beta-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene | Cannabis sativa |
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