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Literature summary extracted from

  • otto, m.; naumann, c.; brandt, w.; wasternack, c.; hause, b.
    activity regulation by heteromerization of Arabidopsis allene oxide cyclase family members (2016), Plants (Basel), 5, pii: E3 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.6 expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.6 K152A/E80A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
5.3.99.6 K152A/E80A/L53S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
5.3.99.6 L53S the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.6 chloroplast
-
Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.99.6 65000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.6 (9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate Arabidopsis thaliana
-
(15Z)-12-oxophyto-10,15-dienoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.6 Arabidopsis thaliana Q9LS02 isoform AOC2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.6 Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.6 (9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate
-
Arabidopsis thaliana (15Z)-12-oxophyto-10,15-dienoate
-
?

Subunits

EC Number Subunits Comment Organism
5.3.99.6 trimer 3 * 26000, isoform AOC2 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
5.3.99.6 Allene oxide cyclase
-
Arabidopsis thaliana
5.3.99.6 AOC2 isoform Arabidopsis thaliana