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Literature summary extracted from

  • Eudes, A.; Sathitsuksanoh, N.; Baidoo, E.E.; George, A.; Liang, Y.; Yang, F.; Singh, S.; Keasling, J.D.; Simmons, B.A.; Loque, D.
    Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency (2015), Plant Biotechnol. J., 13, 1241-1250 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.118 gene qsuB, recombinant expression of the 3-dehydroshikimate dehydratase from Corynebacterium glutamicum in Arabidopsis thaliana transgenic C4H::qsuB lines, the enzyme is targeted to the plastids via plastid-targeting signal peptide (SCHL), reduces lignin content and improves biomass saccharification efficiency, reduction of lignin deposition. Compared to wild-type plants, lines expressing QsuB contain higher amount of protocatechuate, p-coumarate, p-coumaraldehyde and p-coumaryl alcohol, and lower amounts of coniferaldehyde, coniferyl alcohol, sinapaldehyde, and sinapyl alcohol. Metabolite analysis of C4H::qsuB lines, overview. Recombinant expression of YFP-tagged enzyme in plastids of transgenic Arabidopsis thaliana Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.118 3-dehydro-shikimate Corynebacterium glutamicum
-
 protocatechuate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.118 Corynebacterium glutamicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.118 3-dehydro-shikimate
-
 Corynebacterium glutamicum protocatechuate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.118 ? x * 70000, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.118 QsuB
-
 Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
4.2.1.118 metabolism lignin biosynthetic pathway and heterologous expression of bacterial 3-dehydroshikimate dehydratase Corynebacterium glutamicum
4.2.1.118 physiological function the enzyme converts 3-dehydroshikimate, an intermediate of the shikimate pathway, into protocatechuate Corynebacterium glutamicum