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Literature summary extracted from

  • Bencze, L.C.; Filip, A.; Banoczi, G.; Tosa, M.I.; Irimie, F.D.; Gellert, A.; Poppe, L.; Paizs, C.
    Expanding the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum towards styrylalanines (2017), Org. Biomol. Chem., 15, 3717-3727 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.24 F137V the mutant enzyme can transform L-styrylalanine with comparable activity to that of the wild-type enzyme with L-phenylalanine. Ammonia elimination from L-styrylalanine by the non-mutated wild-type enzyme takes place with a 777fold lower kcat/KM value than the deamination of the natural substrate, L-phenylalanine. The mutant enzyme shows enhanced catalytic efficiency in the ammonia elimination reaction of several racemic styrylalanine derivatives Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 0.0783
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.083
-
 L-phenylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.086
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.154
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.186
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.201
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.395
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 1.173
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.3.1.24 1.732
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.3.1.24 4.12
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
4.3.1.24 4.384
-
 L-styrylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 4.969
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Petroselinum crispum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-phenylalanine
-
 Petroselinum crispum trans-cinnamate + NH3
-
 ?
4.3.1.24 L-styrylalanine
-
 Petroselinum crispum (styryl)acrylic acid + NH3
-
 ?
4.3.1.24 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid
-
 Petroselinum crispum (2E,4E)-5-(4-chlorophenyl)penta-2,4-dienoic acid + NH3
-
 ?
4.3.1.24 rac-(E)-2-amino-5-phenylpent-4-enoic acid
-
 Petroselinum crispum (2E,4E)-5-phenylpenta-2,4-dienoic acid + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 30
-
 assay at Petroselinum crispum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.24 0.00024
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.0062
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.0345
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
4.3.1.24 0.0471
-
 L-styrylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.052
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
4.3.1.24 0.0786
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.132
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.3.1.24 0.173
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.276
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.283
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.3.1.24 0.422
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 0.694
-
 L-phenylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8.8
-
 assay at Petroselinum crispum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.1.24 0.0015
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.0083
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
4.3.1.24 0.0104
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137G Petroselinum crispum
4.3.1.24 0.0107
-
 L-styrylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.0156
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, wild-type enzyme Petroselinum crispum
4.3.1.24 0.1125
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.3.1.24 0.163
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137A Petroselinum crispum
4.3.1.24 1.004
-
 rac-(E)-2-amino-5-(4-chlorophenyl)pent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 1.373
-
 rac-(E)-2-amino-5-phenylpent-4-enoic acid pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 2.011
-
 L-phenylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 2.269
-
 L-styrylalanine pH 8.8, 30°C, mutant enzyme F137V Petroselinum crispum
4.3.1.24 8.361
-
 L-phenylalanine pH 8.8, 30°C, wild-type enzyme Petroselinum crispum