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Literature summary extracted from

  • Remus, B.S.; Goldgur, Y.; Shuman, S.
    Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase (2017), Nucleic Acids Res., 45, 12945-12953 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.8 expressed in Escherichia coli BL21-CodonPlus(DE3) cells Candida albicans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.8 in complex with GDP and Mg2+, sitting drop vapor diffusion method, using 0.2 M sodium dihydrogen phosphate, 20% (w/v) PEG3350 Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.8 Mg2+ required Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Candida albicans
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP Candida albicans
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.8 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.8 nickel affinity column chromatography and gel filtration Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Candida albicans (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-aminopurine-riboside-5'-triphosphate about 20% activity with 0.1 mM 2-aminopurine triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 2-aminopurine-riboside-5'-monophosphate + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-mercaptopurine-riboside-5'-triphosphate about 25% activity with 0.1 mM 2-mercaptopurine triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 2-mercaptopurine-riboside-5'-monophosphate + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-chloropurine-riboside-5'-triphosphate about 1% activity with 0.1 mM 6-chloropurine triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 6-chloropurine-riboside-5'-monophosphate + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-O-methylguanosine triphosphate about 45% activity with 0.1 mM 6-O-methyl triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 6-O-methylguanosine phosphate + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ATP about 22% activity with 0.1 mM ATP compared to GTP Candida albicans (ribonucleotide)n+m + AMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + CTP about 18% activity with 0.1 mM CTP compared to GTP Candida albicans (ribonucleotide)n+m + CMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
-
 Candida albicans (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP 100% activity with 0.01 mM GTP Candida albicans (ribonucleotide)n+m + GMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ITP about 75% activity with 0.1 mM ITP compared to GTP Candida albicans (ribonucleotide)n+m + IMP + diphosphate
-
 ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + UTP about 85% activity with 0.1 mM UTP compared to GTP Candida albicans (ribonucleotide)n+m + UMP + diphosphate
-
 ?
6.5.1.8 additional information ATP and CTP are at least 3 orders of magnitude less effective as phosphate donors compared to GTP Candida albicans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.8 Trl1
-
 Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.8 GTP
-
 Candida albicans

General Information

EC Number General Information Comment Organism
6.5.1.8 physiological function fungal tRNA ligase Trl1 is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and noncanonical mRNA splicing in the fungal unfolded protein response Candida albicans