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Literature summary extracted from

  • Wang, P.; Chen, X.L.; Li, C.Y.; Gao, X.; Zhu, D.Y.; Xie, B.B.; Qin, Q.L.; Zhang, X.Y.; Su, H.N.; Zhou, B.C.; Xun, L.Y.; Zhang, Y.Z.
    Structural and molecular basis for the novel catalytic mechanism and evolution of DddP, an abundant peptidase-like bacterial dimethylsulfoniopropionate lyase a new enzyme from an old fold (2015), Mol. Microbiol., 98, 289-301 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.3 expression in Escherichia coli Ruegeria lacuscaerulensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.3 wild-type in complex with inhibitor 2-(N-morpholino)ethanesulfonic acid or PO43-, to 2.1 A resolution, and mutants Y366A and D377N bound to acrylate Ruegeria lacuscaerulensis

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.3 D377A complete loss of activity Ruegeria lacuscaerulensis
4.4.1.3 D377N about 10% residual activity Ruegeria lacuscaerulensis
4.4.1.3 Y366A 20% residual activity Ruegeria lacuscaerulensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.3 1,10-phenanthroline 10 mM, almost complete inhibition Ruegeria lacuscaerulensis
4.4.1.3 2-(N-morpholino)ethanesulfonic acid
-
 Ruegeria lacuscaerulensis
4.4.1.3 bipyridyl 10 mM, 50% inhibition Ruegeria lacuscaerulensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.3 17.1
-
 beta-dimethylsulfoniopropanoate pH 6.0, 60°C Ruegeria lacuscaerulensis

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.3 Ruegeria lacuscaerulensis D0CY07
-
-
4.4.1.3 Ruegeria lacuscaerulensis DSM 11314 D0CY07
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.3 beta-dimethylsulfoniopropanoate
-
 Ruegeria lacuscaerulensis dimethylsulfide + acrylate
-
 ?
4.4.1.3 beta-dimethylsulfoniopropanoate
-
 Ruegeria lacuscaerulensis DSM 11314 dimethylsulfide + acrylate
-
 ?
4.4.1.3 additional information enzyme shows no detectable activity toward the typical substrate (Val-Pro) of M24 peptidases Ruegeria lacuscaerulensis ?
-
 ?
4.4.1.3 additional information enzyme shows no detectable activity toward the typical substrate (Val-Pro) of M24 peptidases Ruegeria lacuscaerulensis DSM 11314 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.3 dddP
-
 Ruegeria lacuscaerulensis
4.4.1.3 SL1157_2466
-
 Ruegeria lacuscaerulensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.3 60
-
-
 Ruegeria lacuscaerulensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.3 6
-
-
 Ruegeria lacuscaerulensis

Expression

EC Number Organism Comment Expression
4.4.1.3 Ruegeria lacuscaerulensis the expression of the dddP gene is significantly induced by DMSP but not by peptide Val-Pro or casein up