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Literature summary extracted from

  • Liechti, G.; Singh, R.; Rossi, P.; Gray, M.; Adams, N.; Maurelli, A.
    Chlamydia trachomatis dapF encodes a bifunctional enzyme capable of both D-glutamate racemase and diaminopimelate epimerase activities (2018), mBio, 9, e00204-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.7 gene dapF, sequence comparisons and phylogenetic analysis, Chlamydia trachomatis dapF restores growth in an Escherichia coli D-glutamate auxotroph strain WM335 also lacking endogenaous gene dapF, expression under the control of an arabinose-inducible promoter, the recombinant enzyme is capable of both DAP epimerase and D-glut racemase activities, DapFCT is also capable of racemizing D-Glu to L-Glu Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.7 LL-2,6-Diaminoheptanedioate Chlamydia trachomatis
-
 meso-Diaminoheptanedioate
-
 r
5.1.1.7 additional information Chlamydia trachomatis Chlamydia trachomatis dapF encodes a bifunctional enzyme capable of both D-glutamate racemase, EC 5.1.1.3, and diaminopimelate epimerase activities. DAP and glutamate appear to be competitive substrates, indicating that they share an active site despite the racemase reaction requiring the pyridoxal 5'-phosphate cofactor ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.7 Chlamydia trachomatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.7 LL-2,6-Diaminoheptanedioate
-
 Chlamydia trachomatis meso-Diaminoheptanedioate
-
 r
5.1.1.7 additional information Chlamydia trachomatis dapF encodes a bifunctional enzyme capable of both D-glutamate racemase, EC 5.1.1.3, and diaminopimelate epimerase activities. DAP and glutamate appear to be competitive substrates, indicating that they share an active site despite the racemase reaction requiring the pyridoxal 5'-phosphate cofactor Chlamydia trachomatis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.7 DapF
-
 Chlamydia trachomatis
5.1.1.7 DapFCT
-
 Chlamydia trachomatis

General Information

EC Number General Information Comment Organism
5.1.1.7 metabolism diaminopimelate epimerase (DapF) catalyzes the final step in the synthesis of meso-diaminopimelate, an amino acid unique to peptidoglycan, and synthesizes D-glutamate, EC 5.1.1.3 Chlamydia trachomatis
5.1.1.7 additional information the structure of Chlamydia DAP epimerase exhibits significant remodeling in the substrate-binding pocket, overview Chlamydia trachomatis