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Literature summary extracted from

  • Wang, S.L.; Li, Y.L.; Han, Z.; Chen, X.; Chen, Q.J.; Wang, Y.; He, L.S.
    Molecular characterization of a novel N-acetylneuraminate lyase from a deep-sea symbiotic Mycoplasma (2018), Mar. Drugs, 16, E80 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.3 EDTA about 120% activity at 5 mM Mycoplasma sp.
4.1.3.3 TritonX-100 about 120% activity at 5 mM Mycoplasma sp.

Application

EC Number Application Comment Organism
4.1.3.3 industry the enzyme has potential in the industrial production of N-acetylneuraminic acid Mycoplasma sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.3 expressed in Escherichia coli BL21(DE3) cells Mycoplasma sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.3 W189A the kcat/Km value of the mutant is about 3fold higher than those of the wild type enzyme Mycoplasma sp.
4.1.3.3 W189F the kcat/Km value of the mutant is about 10fold higher than those of the wild type enzyme Mycoplasma sp.
4.1.3.3 W189Y the kcat/Km value of the mutant is about 7fold higher than those of the wild type enzyme Mycoplasma sp.

General Stability

EC Number General Stability Organism
4.1.3.3 enzyme cleavage activity is measured as 90% of its original activity at 5 MPa and 80% of the activity at 10 MPa Mycoplasma sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 cetyltrimethylammonium bromide 40% inhibition at 5 mM Mycoplasma sp.
4.1.3.3 Fe3+ complete inhibition at 5 mM Mycoplasma sp.
4.1.3.3 SDS 20% inhibition at 5 mM Mycoplasma sp.
4.1.3.3 Zn2+ 30% inhibition at 5 mM Mycoplasma sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 1.8
-
 N-acetylneuraminate at pH 7.0 and 37°C Mycoplasma sp.
4.1.3.3 5.9
-
 pyruvate at pH 7.0 and 37°C Mycoplasma sp.
4.1.3.3 6.9
-
 N-acetyl-D-mannosamine at pH 7.0 and 37°C Mycoplasma sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.3 Ca2+ about 115% activity at 5 mM Mycoplasma sp.
4.1.3.3 K+ about 115% activity at 5 mM Mycoplasma sp.
4.1.3.3 additional information not influenced by Na+ and Mg2+ Mycoplasma sp.
4.1.3.3 Ni2+ about 110% activity at 5 mM Mycoplasma sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.3 N-acetylneuraminate Mycoplasma sp.
-
 N-acetyl-D-mannosamine + pyruvate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Mycoplasma sp. A0A2P1G7E2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-acetyl-D-mannosamine + pyruvate
-
 Mycoplasma sp. N-acetylneuraminate
-
 r
4.1.3.3 N-acetylneuraminate
-
 Mycoplasma sp. N-acetyl-D-mannosamine + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.3 ? x * 52000, SDS-PAGE Mycoplasma sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.3.3 N-Acetylneuraminate lyase
-
 Mycoplasma sp.
4.1.3.3 NAL
-
 Mycoplasma sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.3 65
-
 for N-acetylneuraminate synthesis Mycoplasma sp.
4.1.3.3 70
-
 for N-acetylneuraminate cleavage Mycoplasma sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.3 37 70 the enzyme remains stable after incubation for 150 min at 37°C. When incubated at 70°C, the half-life for enzymatic activity is 30 min and that for a complete loss of activity is 1 h Mycoplasma sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 6 8.5 for N-acetylneuraminate synthesis Mycoplasma sp.
4.1.3.3 7
-
 for N-acetylneuraminate cleavage Mycoplasma sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.3.3 6 8 the enzyme maintains around 95% residual cleavage activity after 120 min incubation at pH 6.0, and 85% residual cleavage activity after 120 min incubation at pH 8.0 Mycoplasma sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.3.3 1.12
-
 pyruvate at pH 7.0 and 37°C Mycoplasma sp.
4.1.3.3 1.86
-
 N-acetyl-D-mannosamine at pH 7.0 and 37°C Mycoplasma sp.
4.1.3.3 3.66
-
 N-acetylneuraminate at pH 7.0 and 37°C Mycoplasma sp.