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Literature summary extracted from

  • Zhu, B.; Ning, L.; Jiang, Y.; Ge, L.
    Biochemical characterization and degradation pattern of a novel endo-type bifunctional alginate lyase AlyA from marine bacterium Isoptericola halotolerans (2018), Mar. Drugs, 16, 258 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Co2+ 10 mM, 75% of initial activity Isoptericola halotolerans
4.2.2.3 Cu2+ 1 mM, 65% of initial activity Isoptericola halotolerans
4.2.2.3 Fe3+ 1 mM, 88% of initial activity Isoptericola halotolerans
4.2.2.3 Ni2+ 1 mM, 87% of initial activity Isoptericola halotolerans
4.2.2.3 Zn2+ 1 mM, 91% of initial activity Isoptericola halotolerans
4.2.2.11 Co2+ 10 mM, 75% of initial activity Isoptericola halotolerans
4.2.2.11 Cu2+ 1 mM, 65% of initial activity Isoptericola halotolerans
4.2.2.11 Fe3+ 1 mM, 88% of initial activity Isoptericola halotolerans
4.2.2.11 Ni2+ 1 mM, 87% of initial activity Isoptericola halotolerans
4.2.2.11 Zn2+ 1 mM, 91% of initial activity Isoptericola halotolerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 3.2
-
 poly(alpha-(1,4)-L-guluronic acid) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 5.6
-
 alginate pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 6.7
-
 poly(beta-(1,4)-D-mannuronic acid) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 3.2
-
 poly(alpha-(1,4)-L-guluronate) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 5.6
-
 alginate pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 6.7
-
 poly(beta-(1,4)-D-mannuronate) pH 7.5, 55°C Isoptericola halotolerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ca2+ 1 mM, 174% of initial activity, 10 mM, 135% of initial activity Isoptericola halotolerans
4.2.2.3 Mg2+ 1 mM, 168% of initial activity, 10 mM, 119% of initial activity Isoptericola halotolerans
4.2.2.3 Na+ 100 mM, 126% of initial activity, 500 mM, 203% of initial activity Isoptericola halotolerans
4.2.2.11 Ca2+ 1 mM, 174% of initial activity, 10 mM, 135% of initial activity Isoptericola halotolerans
4.2.2.11 Mg2+ 1 mM, 168% of initial activity, 10 mM, 119% of initial activity Isoptericola halotolerans
4.2.2.11 Na+ 100 mM, 126% of initial activity, 500 mM, 203% of initial activity Isoptericola halotolerans

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Isoptericola halotolerans A0A346FH09
-
-
4.2.2.3 Isoptericola halotolerans NJ-05 A0A346FH09
-
-
4.2.2.11 Isoptericola halotolerans A0A346FH09
-
-
4.2.2.11 Isoptericola halotolerans NJ-05 A0A346FH09
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 7658
-
 substrate poly(beta-(1,4)-D-mannuronic acid), pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 7985
-
 substrate alginate, pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 8643
-
 substrate poly(alpha-(1,4)-L-guluronic acid), pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 7658
-
 substrate poly(beta-(1,4)-D-mannuronate), pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 7985
-
 substrate alginate, pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 8643
-
 substrate poly(alpha-(1,4)-L-guluronate), pH 7.5, 55°C Isoptericola halotolerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Isoptericola halotolerans unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 alginate
-
 Isoptericola halotolerans NJ-05 unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 poly(alpha-(1,4)-L-guluronic acid) reaction of EC 4.2.2.11 Isoptericola halotolerans 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.3 poly(alpha-(1,4)-L-guluronic acid) reaction of EC 4.2.2.11 Isoptericola halotolerans NJ-05 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.3 poly(beta-(1,4)-D-mannuronic acid)
-
 Isoptericola halotolerans 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
4.2.2.3 poly(beta-(1,4)-D-mannuronic acid)
-
 Isoptericola halotolerans NJ-05 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
4.2.2.11 alginate
-
 Isoptericola halotolerans unsaturated algino-oligosaccharides
-
 ?
4.2.2.11 alginate
-
 Isoptericola halotolerans NJ-05 unsaturated algino-oligosaccharides
-
 ?
4.2.2.11 poly(alpha-(1,4)-L-guluronate)
-
 Isoptericola halotolerans 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.11 poly(alpha-(1,4)-L-guluronate)
-
 Isoptericola halotolerans NJ-05 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.11 poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3 Isoptericola halotolerans 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 25320, calculated from sequence, including His-tag Isoptericola halotolerans
4.2.2.11 ? x * 25320, calculated from sequence, including His-tag Isoptericola halotolerans

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 AlyA
-
 Isoptericola halotolerans
4.2.2.11 AlyA
-
 Isoptericola halotolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 55
-
-
 Isoptericola halotolerans
4.2.2.11 55
-
-
 Isoptericola halotolerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40
-
 30 min, 80% residual activity Isoptericola halotolerans
4.2.2.11 40
-
 30 min, 80% residual activity Isoptericola halotolerans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.3 24.8
-
 poly(alpha-(1,4)-L-guluronic acid) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 26.9
-
 poly(beta-(1,4)-D-mannuronic acid) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 45.9
-
 alginate pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 24.8
-
 poly(alpha-(1,4)-L-guluronate) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 26.9
-
 poly(beta-(1,4)-D-mannuronate) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 45.9
-
 alginate pH 7.5, 55°C Isoptericola halotolerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.5
-
-
 Isoptericola halotolerans
4.2.2.11 7.5
-
-
 Isoptericola halotolerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 5.5 9 24 h, more than 60% resiudal activity Isoptericola halotolerans
4.2.2.11 5.5 9 24 h, more than 60% resiudal activity Isoptericola halotolerans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.3 4
-
 poly(beta-(1,4)-D-mannuronic acid) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 7.6
-
 poly(alpha-(1,4)-L-guluronic acid) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.3 8.2
-
 alginate pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 4
-
 poly(beta-(1,4)-D-mannuronate) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 7.6
-
 poly(alpha-(1,4)-L-guluronate) pH 7.5, 55°C Isoptericola halotolerans
4.2.2.11 8.2
-
 alginate pH 7.5, 55°C Isoptericola halotolerans