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Literature summary extracted from

  • Chen, Z.; Xu, W.; Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.
    Characterization of a thermostable recombinant L-rhamnose isomerase from Caldicellulosiruptor obsidiansis OB47 and its application for the production of L-fructose and L-rhamnulose (2018), J. Sci. Food Agric., 98, 2184-2193 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.1.14 Ca2+ 5% of the activity with Co2+ Caldicellulosiruptor obsidiansis
5.3.1.14 Co2+ best activator Caldicellulosiruptor obsidiansis
5.3.1.14 Fe2+ 21% of the activity with Co2+ Caldicellulosiruptor obsidiansis
5.3.1.14 Mg2+ 34% of the activity with Co2+ Caldicellulosiruptor obsidiansis
5.3.1.14 Mn2+ 95% of the activity with Co2+ Caldicellulosiruptor obsidiansis
5.3.1.14 Ni2+ 39% of the activity with Co2+ Caldicellulosiruptor obsidiansis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 additional information divalent cation required Caldicellulosiruptor obsidiansis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Caldicellulosiruptor obsidiansis D9TIY8
-
-
5.3.1.14 Caldicellulosiruptor obsidiansis OB47 D9TIY8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.14 9.6
-
 substrate L-fructose, pH 8.0, 85°C Caldicellulosiruptor obsidiansis
5.3.1.14 13.7
-
 substrate D-allose, pH 8.0, 85°C Caldicellulosiruptor obsidiansis
5.3.1.14 57.9
-
 substrate L-mannose, pH 8.0, 85°C Caldicellulosiruptor obsidiansis
5.3.1.14 277
-
 substrate L-rhamnose, pH 8.0, 85°C Caldicellulosiruptor obsidiansis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Caldicellulosiruptor obsidiansis D-psicose
-
 r
5.3.1.14 D-allose
-
 Caldicellulosiruptor obsidiansis OB47 D-psicose
-
 r
5.3.1.14 L-fructose
-
 Caldicellulosiruptor obsidiansis L-mannose
-
 r
5.3.1.14 L-fructose
-
 Caldicellulosiruptor obsidiansis OB47 L-mannose
-
 r
5.3.1.14 L-Mannose
-
 Caldicellulosiruptor obsidiansis L-Fructose
-
 r
5.3.1.14 L-Mannose
-
 Caldicellulosiruptor obsidiansis OB47 L-Fructose
-
 r
5.3.1.14 L-Rhamnose
-
 Caldicellulosiruptor obsidiansis L-Rhamnulose
-
 r
5.3.1.14 L-Rhamnose
-
 Caldicellulosiruptor obsidiansis OB47 L-Rhamnulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 48300, claculated from sequence, 4 * 48000, SDS-PAGE Caldicellulosiruptor obsidiansis

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 COB47_0655
-
 Caldicellulosiruptor obsidiansis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 85
-
-
 Caldicellulosiruptor obsidiansis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 70
-
 half-life 24.75 h, presence of Co2+ Caldicellulosiruptor obsidiansis
5.3.1.14 80
-
 half-life 4.15 h, presence of Co2+ Caldicellulosiruptor obsidiansis
5.3.1.14 85
-
 half-life 3.3 h, presence of Co2+ Caldicellulosiruptor obsidiansis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 8
-
-
 Caldicellulosiruptor obsidiansis