BRENDA - Enzyme Database

Expression and properties of the mitochondrial and cytosolic forms of aconitase in maize scutellum

Eprintsev, A.T.; Fedorin, D.N.; Nikitina, M.V.; Igamberdiev, A.U.; J. Plant Physiol. 181, 14-19 (2015)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.3
fumarate
-
Zea mays
4.2.1.3
glyoxylate
-
Zea mays
4.2.1.3
malate
-
Zea mays
4.2.1.3
succinate
-
Zea mays
4.2.1.3
trans-aconitate
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
1.49
-
isocitrate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
1.68
-
isocitrate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
9.5
-
citrate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
21.1
-
citrate
isoform Aco1, pH 8.0, 25°C
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
isoform Aco1; isoform Aco4
Zea mays
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
both isoforms Aco1 and Aco4
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
scutellum
highest activity of both forms of aconitase on the 4th day of germination, then the activity and expression of the cytosolic form sharply decreases, while those of the mitochondrial form decrease more slowly
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
3.95
-
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
4.06
-
isoform Aco1, pH 8.0, 25°C
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
748481
Zea mays
isocitrate
-
-
-
r
4.2.1.3
isocitrate
-
748481
Zea mays
citrate
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
-
mitchondrial isoform Aco1
Zea mays
4.2.1.3
8
-
cytosolic isoform Aco4
Zea mays
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.3
0.18
-
trans-aconitate
substrate isocitrate, isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.25
-
trans-aconitate
substrate isocitrate, isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.26
-
trans-aconitate
substrate citrate, isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.39
-
trans-aconitate
substrate citrate, isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.41
-
fumarate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.58
-
fumarate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.58
-
succinate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.68
-
glyoxylate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.68
-
succinate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.71
-
malate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.72
-
glyoxylate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.95
-
malate
isoform Aco4, pH 8.0, 25°C
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.3
fumarate
-
Zea mays
4.2.1.3
glyoxylate
-
Zea mays
4.2.1.3
malate
-
Zea mays
4.2.1.3
succinate
-
Zea mays
4.2.1.3
trans-aconitate
-
Zea mays
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.3
0.18
-
trans-aconitate
substrate isocitrate, isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.25
-
trans-aconitate
substrate isocitrate, isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.26
-
trans-aconitate
substrate citrate, isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.39
-
trans-aconitate
substrate citrate, isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.41
-
fumarate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.58
-
fumarate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.58
-
succinate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.68
-
glyoxylate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.68
-
succinate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.71
-
malate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
0.72
-
glyoxylate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
0.95
-
malate
isoform Aco4, pH 8.0, 25°C
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
1.49
-
isocitrate
isoform Aco1, pH 8.0, 25°C
Zea mays
4.2.1.3
1.68
-
isocitrate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
9.5
-
citrate
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
21.1
-
citrate
isoform Aco1, pH 8.0, 25°C
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
isoform Aco1; isoform Aco4
Zea mays
5829
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
both isoforms Aco1 and Aco4
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
scutellum
highest activity of both forms of aconitase on the 4th day of germination, then the activity and expression of the cytosolic form sharply decreases, while those of the mitochondrial form decrease more slowly
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
3.95
-
isoform Aco4, pH 8.0, 25°C
Zea mays
4.2.1.3
4.06
-
isoform Aco1, pH 8.0, 25°C
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
748481
Zea mays
isocitrate
-
-
-
r
4.2.1.3
isocitrate
-
748481
Zea mays
citrate
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
-
mitchondrial isoform Aco1
Zea mays
4.2.1.3
8
-
cytosolic isoform Aco4
Zea mays