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Literature summary extracted from

  • Eprintsev, A.T.; Fedorin, D.N.; Nikitina, M.V.; Igamberdiev, A.U.
    Expression and properties of the mitochondrial and cytosolic forms of aconitase in maize scutellum (2015), J. Plant Physiol., 181, 14-19 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.3 fumarate
-
 Zea mays
4.2.1.3 glyoxylate
-
 Zea mays
4.2.1.3 malate
-
 Zea mays
4.2.1.3 succinate
-
 Zea mays
4.2.1.3 trans-aconitate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.3 1.49
-
 isocitrate isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 1.68
-
 isocitrate isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 9.5
-
 citrate isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 21.1
-
 citrate isoform Aco1, pH 8.0, 25°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.3 cytosol isoform Aco1 Zea mays 5829
-
4.2.1.3 cytosol isoform Aco4 Zea mays 5829
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.3 both isoforms Aco1 and Aco4 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.3 scutellum highest activity of both forms of aconitase on the 4th day of germination, then the activity and expression of the cytosolic form sharply decreases, while those of the mitochondrial form decrease more slowly Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.3 3.95
-
 isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 4.06
-
 isoform Aco1, pH 8.0, 25°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.3 citrate
-
 Zea mays isocitrate
-
 r
4.2.1.3 isocitrate
-
 Zea mays citrate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.3 Aco1 mitochondrial isoform Zea mays
4.2.1.3 ACO4 cytosolic isoform Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.3 7.5
-
 mitchondrial isoform Aco1 Zea mays
4.2.1.3 8
-
 cytosolic isoform Aco4 Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.3 0.18
-
 trans-aconitate substrate isocitrate, isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 0.25
-
 trans-aconitate substrate isocitrate, isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 0.26
-
 trans-aconitate substrate citrate, isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 0.39
-
 trans-aconitate substrate citrate, isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 0.41
-
 fumarate isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 0.58
-
 succinate isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 0.58
-
 fumarate isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 0.68
-
 glyoxylate isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 0.68
-
 succinate isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 0.71
-
 malate isoform Aco1, pH 8.0, 25°C Zea mays
4.2.1.3 0.72
-
 glyoxylate isoform Aco4, pH 8.0, 25°C Zea mays
4.2.1.3 0.95
-
 malate isoform Aco4, pH 8.0, 25°C Zea mays