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Literature summary extracted from

  • Camps Bres, F.; Guerard-Hélaine, C.; Hélaine, V.; Fernandes, C.; Sanchez-Moreno, I.; Traikia, M.; Garcia-Junceda, E.; Lemaire, M.
    L-Rhamnulose-1-phosphate and L-fuculose-1-phosphate aldolase mediated multi-enzyme cascade systems for nitrocyclitol synthesis (2015), J. Mol. Catal. B, 114, 50-57 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.17 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
4.1.2.19 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.17 L-fuculose 1-phosphate Escherichia coli
-
 glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 L-Rhamnulose 1-phosphate Escherichia coli
-
 Glycerone phosphate + (S)-lactaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.17 Escherichia coli P0AB87
-
-
4.1.2.19 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.17 Ni-NTA column chromatography Escherichia coli
4.1.2.19 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.17 (2R)-2,3-dihydroxy-4-nitrobutanal
-
 Escherichia coli (1S,2R,3R,4R,5R,6R)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol + (1R,2R,3R,4R,5S,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol
-
 ?
4.1.2.17 (2S)-2,3-dihydroxy-4-nitrobutanal
-
 Escherichia coli (1R,2R,3R,4S,5S,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol + (1S,2R,3S,4S,5R,6R)-1-(hydroxymethyl)-6-nitrocyclohexane-1,2,3,4,5-pentol
-
 ?
4.1.2.17 3-hydroxy-4-nitrobutanal
-
 Escherichia coli (1S,2R,3R,5S,6R)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,5-tetraol + (1R,2R,3R,5R,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,5-tetraol
-
 ?
4.1.2.17 4-nitrobutanal
-
 Escherichia coli (1R,2R,3S,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3-triol + (1S,2R,3R,6R)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3-triol
-
 ?
4.1.2.17 dimethoxyacetaldehyde + glycerone phosphate
-
 Escherichia coli (3R,4S)-3,4-dihydroxy-5,5-dimethoxy-2-oxopentyl phosphate
-
 ?
4.1.2.17 L-fuculose 1-phosphate
-
 Escherichia coli glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.19 (2R)-2,3-dihydroxy-4-nitrobutanal
-
 Escherichia coli (1R,2R,3S,4R,5S,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol + (1S,2R,3R,4R,5R,6R)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol
-
 r
4.1.2.19 (2S)-2,3-dihydroxy-4-nitrobutanal
-
 Escherichia coli (1R,2R,3S,4S,5S,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol + 1-hydroxymethyl-6-nitrocyclohexane-1,2,3,4,5-pentol
-
 r
4.1.2.19 3-hydroxy-4-nitrobutanal
-
 Escherichia coli (1R,2R,3S,5R,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,5-tetraol + (1S,2R,3R,5S,6R)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3,5-tetraol
-
 r
4.1.2.19 4-nitrobutanal
-
 Escherichia coli (1R,2R,3S,6S)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3-triol + (1S,2R,3R,6R)-1-hydroxymethyl-6-nitrocyclohexane-1,2,3-triol
-
 ?
4.1.2.19 dimethoxyacetaldehyde + glycerone phosphate
-
 Escherichia coli (3R,4R)-3,4-dihydroxy-5,5-dimethoxy-2-oxopentyl phosphate
-
 ?
4.1.2.19 L-Rhamnulose 1-phosphate
-
 Escherichia coli Glycerone phosphate + (S)-lactaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.17 FucA
-
 Escherichia coli
4.1.2.17 L-fuculose-1-phosphate aldolase
-
 Escherichia coli
4.1.2.19 L-Rhamnulose-1-phosphate aldolase
-
 Escherichia coli
4.1.2.19 RhuA
-
 Escherichia coli