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Literature summary extracted from

  • Chen, J.; Zhu, Y.; Fu, G.; Song, Y.; Jin, Z.; Sun, Y.; Zhang, D.
    High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis (2016), J. Ind. Microbiol. Biotechnol., 43, 1577-1591 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.30 gene rdpe, recombinant constitutive enzyme expression in Bacillus subtilis strains 1A751-SR, 1A751-GR, and 1A751S1-XR (from strain 1A751), in which two extracellular proteases (nprE, aprE) are deleted, Bacillus subtilis is transformed using the Paris method, screening and characterization of different inducible promoters, xylose-inducible expression system, method evaluation, detailed overview Ruminococcus sp. 5_1_39BFAA

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.30 additional information high-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis, fed-batch fermentation in 7.5 l fermentor, overview Ruminococcus sp. 5_1_39BFAA

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.30 D-psicose Ruminococcus sp. 5_1_39BFAA
-
 D-fructose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.30 Ruminococcus sp. 5_1_39BFAA
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.30 D-psicose
-
 Ruminococcus sp. 5_1_39BFAA D-fructose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.30 DPEase
-
 Ruminococcus sp. 5_1_39BFAA
5.1.3.30 RDPE
-
 Ruminococcus sp. 5_1_39BFAA

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.30 55
-
 assay at Ruminococcus sp. 5_1_39BFAA

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.30 8
-
 assay at Ruminococcus sp. 5_1_39BFAA

Expression

EC Number Organism Comment Expression
5.1.3.30 Ruminococcus sp. 5_1_39BFAA xylose induces the expression of recombinant RDPE up

General Information

EC Number General Information Comment Organism
5.1.3.30 physiological function D-psicose 3-epimerase (DPEase) converts D-fructose into d-psicose which exists in nature in limited quantities and has key physiological functions Ruminococcus sp. 5_1_39BFAA