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Literature summary extracted from

  • Soydan, E.; Gueler, A.; Biyik, S.; Sentuerk, M.; Supuran, C.T.; Ekinci, D.
    Carbonic anhydrase from Apis mellifera purification and inhibition by pesticides (2017), J. Enzyme Inhib. Med. Chem., 32, 47-50 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 Atrazine
-
 Apis mellifera
4.2.1.1 Carbaryl
-
 Apis mellifera
4.2.1.1 carbofuran
-
 Apis mellifera
4.2.1.1 propoxur
-
 Apis mellifera
4.2.1.1 simazine
-
 Apis mellifera
4.2.1.1 tebuconazole
-
 Apis mellifera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ required, metalloenzyme Apis mellifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Apis mellifera
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Apis mellifera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 native enzyme 35fold from spermatheca by L-tyrosine-sulfanylamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3 Apis mellifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 spermatheca
-
 Apis mellifera
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
 175 EU/mg protein activity in CO2 hydration reaction, purified native enzyme, pH and temperature not specified in the publication Apis mellifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Apis mellifera H2CO3
-
 r
4.2.1.1 H2CO3
-
 Apis mellifera CO2 + H2O
-
 r
4.2.1.1 additional information the enzyme is also active in an esterase assay with 4-nitrophenylacetate as substrate Apis mellifera ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 ? x * 32000, SDS-PAGE Apis mellifera

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 Am CA
-
 Apis mellifera
4.2.1.1 AmCA
-
 Apis mellifera

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 additional information
-
 AmCA inhibition by the pesticides is also measured (IC50 values) using an esterase assay with 4-nitrophenylacetate as substrate Apis mellifera additional information
4.2.1.1 0.003
-
 measured with hydration of CO2 activity assay, pH and temperature not specified in the publication Apis mellifera tebuconazole
4.2.1.1 0.0031
-
 measured with hydration of CO2 activity assay, pH and temperature not specified in the publication Apis mellifera Carbaryl
4.2.1.1 0.0087
-
 measured with hydration of CO2 activity assay, pH and temperature not specified in the publication Apis mellifera carbofuran
4.2.1.1 0.0165
-
 measured with hydration of CO2 activity assay, pH and temperature not specified in the publication Apis mellifera Atrazine
4.2.1.1 0.0273
-
 measured with hydration of CO2 activity assay, pH and temperature not specified in the publication Apis mellifera simazine
4.2.1.1 0.0321
-
 measured with hydration of CO2 activity assay, pH and temperature not specified in the publication Apis mellifera propoxur