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Literature summary extracted from

  • Inoue, A.; Anraku, M.; Nakagawa, S.; Ojima, T.
    Discovery of a novel alginate lyase from Nitratiruptor sp. SB155-2 thriving at deep-sea hydrothermal vents and identification of the residues responsible for its heat stability (2016), J. Biol. Chem., 291, 15551-15563 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression in Escherichia coli Nitratiruptor sp. SB155-2

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.3 additional information introduction of two cysteines based on homology modeling using Pseudomonas aeruginosa alginate lyase PA1167 as the template enhances heat stability Nitratiruptor sp. SB155-2

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Fe2+ 1 mM, 69% inhibition Nitratiruptor sp. SB155-2
4.2.2.3 Fe3+ 1 mM, 86% inhibition Nitratiruptor sp. SB155-2
4.2.2.3 Pb2+ 1 mM, 82% inhibition Nitratiruptor sp. SB155-2
4.2.2.3 Sn2+ 1 mM, 85% inhibition Nitratiruptor sp. SB155-2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Na+ 0.8-1.4 M NaCl required for maximum activity Nitratiruptor sp. SB155-2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 27000
-
 PAGE Nitratiruptor sp. SB155-2

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Nitratiruptor sp. SB155-2 A6Q1E0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 1620
-
 substrate poly(beta-1,4-D-mannuronate), pH 6.0, 70°C Nitratiruptor sp. SB155-2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate about 30% of the activity with poly(beta-1,4-D-mannuronate) Nitratiruptor sp. SB155-2 unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 poly(alpha-1,4-L-guluronate) about 5% of the activity with poly(beta-1,4-D-mannuronate), reaction of EC 4.2.2.11 Nitratiruptor sp. SB155-2 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate)
-
 ?
4.2.2.3 poly(beta-1,4-D-mannuronate) prefered substrate Nitratiruptor sp. SB155-2 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate)
-
 ?
4.2.2.3 poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate) about 30% of the activity with poly(beta-1,4-D-mannuronate) Nitratiruptor sp. SB155-2 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate)
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 26692, calculated from sequence, 1 * 29000, SDS-PAGE Nitratiruptor sp. SB155-2

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 NIS_0185
-
 Nitratiruptor sp. SB155-2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 70
-
-
 Nitratiruptor sp. SB155-2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 additional information
-
 heat stability is decreased in the presence of 5 mM dithiotreitol, and residues Cys80 and Cys232 are responsible for heat stability but not lyase activity Nitratiruptor sp. SB155-2
4.2.2.3 67
-
 30 min, 50% loss of activity Nitratiruptor sp. SB155-2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 6
-
-
 Nitratiruptor sp. SB155-2

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 5 6.5 more than 80% of maximum activity Nitratiruptor sp. SB155-2