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Literature summary extracted from

  • Miles, Z.D.; Roberts, S.A.; McCarty, R.M.; Bandarian, V.
    Biochemical and structural studies of 6-carboxy-5,6,7,8-tetrahydropterin synthase reveal the molecular basis of catalytic promiscuity within the tunnel-fold superfamily (2014), J. Biol. Chem., 289, 23641-23652 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.50
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.50 hanging drop vapor diffusion method, using 5% (v/v) 2-methyl-1,3-propanediol, 0.04 M CaCl2, 0.1 M sodium acetate (pH 4.5) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.50 C27A inactive Escherichia coli
4.1.2.50 D70N/H71A the mutant shows greatly reduced activity compared to the wild type enzyme Escherichia coli
4.1.2.50 H25A/D54N inactive Escherichia coli
4.1.2.50 H25A/D54N/D70N/H71A inactive Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.50 Zn2+ contains a catalytic zinc ion Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.50 7,8-dihydroneopterin 3'-triphosphate + H2O Escherichia coli
-
 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.50 Escherichia coli P65870
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.50
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.50 7,8-dihydroneopterin 3'-triphosphate + H2O
-
 Escherichia coli 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate
-
 ?
4.1.2.50 sepiapterin + H2O
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.50 homohexamer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.1.2.50 6-carboxy-5,6,7,8-tetrahydropterin synthase
-
 Escherichia coli
4.1.2.50 6-pyruvoyltetrahydropterin synthase
-
 Escherichia coli
4.1.2.50 CPH4 synthase
-
 Escherichia coli
4.1.2.50 PTPS
-
 Escherichia coli
4.1.2.50 QueD
-
 Escherichia coli