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Literature summary extracted from
- Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean (1989), J. Biol. Chem., 264, 20068-20073 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | Phospholipid | the enzyme reaction is stimulated by exogenous phospholipid in the presence of detergent | Vigna radiata |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | ATP | more than 80% inhibition at 2 mM | Vigna radiata | |
| 7.1.3.1 | CTP | more than 80% inhibition at 2 mM | Vigna radiata | |
| 7.1.3.1 | diphosphate | excess of diphosphate inhibits the enzyme | Vigna radiata | |
| 7.1.3.1 | fluoride | inhibitory at high concentrations | Vigna radiata | |
| 7.1.3.1 | GTP | more than 80% inhibition at 2 mM | Vigna radiata | |
| 7.1.3.1 | Mg2+ | excess of Mg2+ inhibits the enzyme | Vigna radiata | |
| 7.1.3.1 | additional information | nitrate (50 mM), molybdate (0.5 mM), and azide (2 mM) have essentially no effect on the enzyme reaction | Vigna radiata | |
| 7.1.3.1 | N,N'-dicyclohexylcarbodiimide | - |
Vigna radiata | |
| 7.1.3.1 | vanadate | inhibitory at high concentrations | Vigna radiata | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.3.1 | tonoplast | - |
Vigna radiata | - |
- |
| 7.1.3.1 | vacuolar membrane | - |
Vigna radiata | 5774 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | K+ | for maximum activity, the purified enzyme requires 50 mM K+ | Vigna radiata | |
| 7.1.3.1 | Mg2+ | for maximum activity, the purified enzyme requires 1 mM Mg2+ | Vigna radiata | |
| 7.1.3.1 | Mn2+ | slight stimulatory effect at 1 mM Mn2+ | Vigna radiata | |
| 7.1.3.1 | additional information | Co2+ and Ca2+ have no stimulatory effect on the enzyme activity | Vigna radiata |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Vigna radiata | - |
2 phosphate + H+[side 2] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.3.1 | Vigna radiata | - |
cultivar Wilczek | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.3.1 | solubilization with lysophosphatidylcholine and QAE-Toyopearl column chromatography | Vigna radiata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.1.3.1 | hypocotyl | - |
Vigna radiata | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.3.1 | ? | x * 73000, SDS-PAGE | Vigna radiata |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.3.1 | vacuolar membrane proton-translocating inorganic pyrophosphatase | - |
Vigna radiata |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 7.1.3.1 | 2 | - |
pH and temperature not specified in the publication | Vigna radiata | vanadate | |
| 7.1.3.1 | 5 | - |
pH and temperature not specified in the publication | Vigna radiata | fluoride | |
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