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Literature summary extracted from

  • Kocyigit, U.M.; Taslimi, P.; Gulcin, I.
    Characterization and inhibition effects of some metal ions on carbonic anhydrase enzyme from Kangal Akkaraman sheep (2018), J. Biochem. Mol. Toxicol., 32, e22172 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 Ag+ uncompetitive inhibition Ovis aries
4.2.1.1 Co2+ uncompetitive inhibition Ovis aries
4.2.1.1 Cu2+ uncompetitive inhibition Ovis aries
4.2.1.1 Fe2+ uncompetitive inhibition Ovis aries
4.2.1.1 Hg2+ uncompetitive inhibition Ovis aries
4.2.1.1 Pb2+ uncompetitive inhibition Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 additional information
-
 additional information Lineweaver-Burk kinetics Ovis aries

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ metalloenzyme Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Ovis aries
-
 CO2 + H2O
-
 r
4.2.1.1 H2CO3 Ovis aries Kangal Akkaraman
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Ovis aries
-
-
-
4.2.1.1 Ovis aries Kangal Akkaraman
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 native enzynme from erythrocytes, 203fold by L-tyrosine sulfanilamide affinity chromatography Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 erythrocyte
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
 6681.57 EU/mg, purified enzyme, pH 7.4, 25°C, p-nitrophenyl acetate substrate Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Ovis aries CO2 + H2O
-
 r
4.2.1.1 H2CO3
-
 Ovis aries Kangal Akkaraman CO2 + H2O
-
 r
4.2.1.1 additional information enzyme esterase activity assay using p-nitrophenyl acetate as substrate Ovis aries ?
-
 ?
4.2.1.1 additional information enzyme esterase activity assay using p-nitrophenyl acetate as substrate Ovis aries Kangal Akkaraman ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 ? x * 28890, SDS-PAGE Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 30
-
-
 Ovis aries

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.1
-
 60 activity range, profile overview Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 7.5
-
-
 Ovis aries

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 5 10 activity range, profile overview Ovis aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.12
-
 Pb2+ pH 7.4, 25°C, purified enzyme Ovis aries
4.2.1.1 0.401
-
 Co2+ pH 7.4, 25°C, purified enzyme Ovis aries
4.2.1.1 0.543
-
 Hg2+ pH 7.4, 25°C, purified enzyme Ovis aries
4.2.1.1 0.571
-
 Cu2+ pH 7.4, 25°C, purified enzyme Ovis aries
4.2.1.1 0.915
-
 Fe2+ pH 7.4, 25°C, purified enzyme Ovis aries
4.2.1.1 1.36
-
 Ag+ pH 7.4, 25°C, purified enzyme Ovis aries

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.11
-
 pH 7.4, 25°C, purified enzyme Ovis aries Pb2+
4.2.1.1 0.4
-
 pH 7.4, 25°C, purified enzyme Ovis aries Hg2+
4.2.1.1 0.42
-
 pH 7.4, 25°C, purified enzyme Ovis aries Co2+
4.2.1.1 0.63
-
 pH 7.4, 25°C, purified enzyme Ovis aries Cu2+
4.2.1.1 1.15
-
 pH 7.4, 25°C, purified enzyme Ovis aries Ag+
4.2.1.1 1.22
-
 pH 7.4, 25°C, purified enzyme Ovis aries Fe2+