EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.14 | expressed in Escherichia coli and Haloferax volcanii H1209 | Haloferax volcanii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.14 | 0.29 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at pH 7.0 and 42°C | Haloferax volcanii | |
4.1.2.14 | 38 | - |
D-glyceraldehyde 3-phosphate | at pH 7.0 and 42°C | Haloferax volcanii | |
4.2.1.39 | 3 | - |
D-gluconate | pH 7.5, 42°C | Haloferax volcanii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.14 | K+ | maximal activities at concentrations of between 1 and 1.5 M KCl | Haloferax volcanii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.14 | 267000 | - |
gel filtration | Haloferax volcanii |
4.2.1.39 | 340000 | - |
gel filtration | Haloferax volcanii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | Haloferax volcanii | - |
pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | Haloferax volcanii H26 | - |
pyruvate + D-glyceraldehyde 3-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.14 | Haloferax volcanii | D4GV57 | - |
- |
4.1.2.14 | Haloferax volcanii H26 | D4GV57 | - |
- |
4.2.1.39 | Haloferax volcanii | D4GYD4 | - |
- |
4.2.1.39 | Haloferax volcanii DSM 3757 | D4GYD4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.14 | Ni-NTA column chromatography and Superdex 200 gel filtration | Haloferax volcanii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.39 | 30 | - |
pH 7.5, 42°C | Haloferax volcanii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-galactonate | low activity | Haloferax volcanii | ? | - |
? | |
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-galactonate | low activity | Haloferax volcanii H26 | ? | - |
? | |
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Haloferax volcanii | pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | 100% activity | Haloferax volcanii | pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Haloferax volcanii H26 | pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | 100% activity | Haloferax volcanii H26 | pyruvate + D-glyceraldehyde 3-phosphate | - |
r | |
4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate | about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate | Haloferax volcanii | pyruvate + D-glyceraldehyde | - |
? | |
4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate | about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate | Haloferax volcanii H26 | pyruvate + D-glyceraldehyde | - |
? | |
4.1.2.14 | pyruvate + D-glyceraldehyde 3-phosphate | - |
Haloferax volcanii | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
r | |
4.1.2.14 | pyruvate + D-glyceraldehyde 3-phosphate | - |
Haloferax volcanii H26 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
r | |
4.2.1.39 | D-galactonate | low activity | Haloferax volcanii | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
4.2.1.39 | D-galactonate | low activity | Haloferax volcanii DSM 3757 | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
4.2.1.39 | D-gluconate | - |
Haloferax volcanii | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.39 | D-gluconate | - |
Haloferax volcanii DSM 3757 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.39 | additional information | the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates | Haloferax volcanii | ? | - |
? | |
4.2.1.39 | additional information | the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates | Haloferax volcanii DSM 3757 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.14 | homododecamer | 12 * 22400, calculated from amino acid sequence | Haloferax volcanii |
4.2.1.39 | octamer | 8 * 45800, calculated from sequence | Haloferax volcanii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.14 | 2-keto-3-deoxy-6-phosphogluconate aldolase | - |
Haloferax volcanii |
4.1.2.14 | KDPG aldolase | - |
Haloferax volcanii |
4.1.2.14 | KDPGA | - |
Haloferax volcanii |
4.2.1.39 | GAD | - |
Haloferax volcanii |
4.2.1.39 | HVO_1488 | - |
Haloferax volcanii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.14 | 7 | - |
- |
Haloferax volcanii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.14 | 5.5 | 9 | 50% activity between pH 5.5 and 9.0 | Haloferax volcanii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.2.14 | metabolism | the enzyme is essential for glucose degradation | Haloferax volcanii |
4.2.1.39 | physiological function | a knockout mutant of GAD loses the ability to grow on glucose. Following a prolonged incubation period, GAD is functionally replaced by xylonate dehydratase | Haloferax volcanii |