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Literature summary extracted from

  • Zhang, P.; Shao, Z.; Li, L.; Liu, S.; Yao, J.; Duan, D.
    Molecular characterisation and biochemical properties of phosphomannomutase/phosphoglucomutase (PMM/PGM) in the brown seaweed Saccharina japonica (2018), J. Appl. Phycol., 30, 2687-2696 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.2 alpha-D-Glucose 1,6-bisphosphate dependent on Saccharina japonica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expressed as phosphomannomutase/phosphoglucomutase fusion protein in Escherichia coli Transetta (DE3) cells Saccharina japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.2 additional information not inhibited by NaCl or KCl Saccharina japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.08
-
 alpha-D-glucose 1-phosphate at pH 7.0 and 30°C Saccharina japonica
5.4.2.2 1.15
-
 alpha-D-mannose 1-phosphate at pH 7.5 and 30°C Saccharina japonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+ maximum activity in the presence of Mg2+ Saccharina japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate Saccharina japonica
-
 D-glucose 6-phosphate
-
 ?
5.4.2.2 alpha-D-mannose 1-phosphate Saccharina japonica
-
 D-mannose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Saccharina japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 maltose binding protein-Trap column chromatography and Superdex 200 gel filtration Saccharina japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.2 sporophyte
-
 Saccharina japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Saccharina japonica D-glucose 6-phosphate
-
 ?
5.4.2.2 alpha-D-mannose 1-phosphate
-
 Saccharina japonica D-mannose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 phosphomannomutase/phosphoglucomutase bifunctional enzyme Saccharina japonica
5.4.2.2 PMM/PGM bifunctional enzyme Saccharina japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.2 30
-
-
 Saccharina japonica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.2.2 25 35 more than 70% activity between 25 and 35°C Saccharina japonica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.2 30 60 the enzyme is stable at 30°C (compared to other temperatures) but loses most of its activity after incubation at 50 and 60°C for 10 min Saccharina japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.2 7
-
 pH optimum for phosphomannomutase activity Saccharina japonica
5.4.2.2 7.5
-
 pH optimum for phosphoglucomutase activity Saccharina japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.2.2 6 7.5 more than 50% activity between pH 6.0 and 7.5 Saccharina japonica

Expression

EC Number Organism Comment Expression
5.4.2.2 Saccharina japonica high-temperature (25°C) and high-light intensity (0.055 mM photons m-2 s-1) both significantly increase the transcription of the enzyme 4.6fold up