Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Z.; Zhuang, W.; Cheng, J.; Sun, W.; Wu, J.; Chen, Y.; Ying, H.
    In vivo multienzyme complex coconstruction of N-acetylneuraminic acid lyase and N-acetylglucosamine-2-epimerase for biosynthesis of N-acetylneuraminic acid (2017), J. Agric. Food Chem., 65, 7467-7475 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.3 expressed in Escherichia coli Rosetta cells Corynebacterium glutamicum
5.1.3.8 recombinant enzyme expression in Escherichia coli, coexpression with N-acetylneuraminic acid lyase from Corynebacterium glutamicum ATCC 13032 (CgNal, EC 4.1.3.3) Anabaena sp. CH1

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.8 additional information metabolic channeling enables efficient transfer of the intermediates by forming a multienzyme complex. To leverage the metabolic channeling for improved biosynthesis, N-acetylneuraminic acid lyase from Corynebacterium glutamicum ATCC 13032 (CgNal, EC 4.1.3.3) and N-acetylglucosamine-2-epimerase from Anabaena sp. CH1 (anAGE) are coexpressed in Escherichia coli and the whole cell are used to synthesize N-acetylneuraminic acid (Neu5Ac) from N-acetylglucosamine (GlcNAc) and pyruvate. To get the multienzyme complex, a polycistronic plasmid with high levels of CgNal and anAGE expression is constructed by tuning the orders of the genes. The Shine-Dalgarno (SD) sequence and aligned spacing (AS) distance are optimized. The Escherichia coli strain Rosetta harboring the polycistronic plasmid pET-28a-SD2-AS1-CgNal-SD-AS-anAGE increases the production of Neu5Ac by 58.7% to 92.5 g/l in 36 h by whole-cell catalysis and by 21.9% up to 112.8 g/l in 24 h with the addition of Triton X-100 Anabaena sp. CH1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.3 N-acetylneuraminate Corynebacterium glutamicum
-
 N-acetyl-D-mannosamine + pyruvate
-
 r
4.1.3.3 N-acetylneuraminate Corynebacterium glutamicum ATCC 13032
-
 N-acetyl-D-mannosamine + pyruvate
-
 r
5.1.3.8 N-Acyl-D-glucosamine Anabaena sp. CH1
-
 N-Acyl-D-mannosamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Corynebacterium glutamicum
-
-
-
4.1.3.3 Corynebacterium glutamicum ATCC 13032
-
-
-
5.1.3.8 Anabaena sp. CH1 A4UA16
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-acetylneuraminate
-
 Corynebacterium glutamicum N-acetyl-D-mannosamine + pyruvate
-
 r
4.1.3.3 N-acetylneuraminate
-
 Corynebacterium glutamicum ATCC 13032 N-acetyl-D-mannosamine + pyruvate
-
 r
5.1.3.8 N-Acyl-D-glucosamine
-
 Anabaena sp. CH1 N-Acyl-D-mannosamine
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.3.3 NAL
-
 Corynebacterium glutamicum
5.1.3.8 AGE
-
 Anabaena sp. CH1
5.1.3.8 anAGE
-
 Anabaena sp. CH1