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Literature summary extracted from
- Leishmania donovani impairment of the cellular immune response against recombinant ornithine decarboxylase protein as a possible evasion strategy of Leishmania in visceral leishmaniasis (2015), Int. J. Parasitol., 45, 33-42 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.1.17 | drug development | because ODC is essential for trypanothione biosynthesis, polyamines of parasitic protozoa may be potential targets for the development of drugs with activity against Leishmania donovani. One goal of drug development is to evaluate the potential of Leishmania antigen for evoking a Th1 response against Leishmania infection | Leishmania donovani |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.17 | gene odc, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 | Leishmania donovani |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.17 | L-alpha-difluoromethylornithine | - |
Leishmania donovani |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.17 | L-ornithine | Leishmania donovani | - |
putrescine + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.17 | Leishmania donovani | P27116 | prepared from a Leishmania donovani clinical strain procured from a patient admitted to the Rajendra Medical Research Institute of Medical Science, Patna, India as described by Gupta et al. (2007) | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.17 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis | Leishmania donovani |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.1.17 | amastigote | - |
Leishmania donovani | - |
| 4.1.1.17 | additional information | significantly higher growth of Leishmania donovani parasites in the presence of recombinant LdODC compared with the parasite culture in the absence of any stimulation of both promastigotes and amastigotes, although it is higher in amastigotes | Leishmania donovani | - |
| 4.1.1.17 | promastigote | - |
Leishmania donovani | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.17 | L-ornithine | - |
Leishmania donovani | putrescine + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.17 | ? | x * 77000, about, recombinant His-tagged enzyme, SDS-PAGE | Leishmania donovani |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.17 | LdODC | - |
Leishmania donovani |
| 4.1.1.17 | ODC | - |
Leishmania donovani |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.17 | pyridoxal 5'-phosphate | - |
Leishmania donovani | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.1.17 | malfunction | use of enzyme inhibitor alpha-difluoromethylornithine can reduce the activity of ornithine decarboxylase of Leishmannia donovani eliminating the parasite-induced immune suppression and inducing collateral host protective responses in visceral leishmaniasis. Inhibition of r-LdODC can direct the active IFN-c dominant immune responses necessary for parasite clearance in VL patients | Leishmania donovani |
| 4.1.1.17 | physiological function | ODC is essential for trypanothione biosynthesis. Trypanothione is the major reduced thiol which is responsible for the modulation of the immune response and pathogenesis in visceral leishmaniasis (VL). Analysis of the host protective role of an anti-ODC CD4+ T cell response and anti-leishmanial MU function in VL patients, relationship between ornithine decarboxylase and the cellular immune response in VL patients, overview. Pivotal role for recombinant LdODC in causing strong immune suppression in a susceptible host. Recombinant LdODC-induced CD4+ and CD8+ T-cell responses, functional profiles and effects on IFN-gamma and interleukin-10 levels, overview | Leishmania donovani |
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