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Literature summary extracted from

  • de Oliveira, G.; Adriani, P.; Borges, F.; Lopes, A.; Campana, P.; Chambergo, F.
    Epoxide hydrolase of Trichoderma reesei Biochemical properties and conformational characterization (2016), Int. J. Biol. Macromol., 89, 569-574 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.10 expression in Escherichia coli Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 3
-
 (S)-styrene oxide pH 7.0, 37°C Trichoderma reesei
3.3.2.10 21.8
-
 (R)-styrene oxide pH 7.0, 37°C Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 38200
-
 calculated from sequence Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Trichoderma reesei G0R7E2
-
-
3.3.2.10 Trichoderma reesei QM9414 G0R7E2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 (R)-styrene oxide + H2O low activity, the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei (R)-styrene glycol
-
 ?
3.3.2.10 (R)-styrene oxide + H2O low activity, the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei QM9414 (R)-styrene glycol
-
 ?
3.3.2.10 (S)-styrene oxide + H2O the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei (S)-styrene glycol
-
 ?
3.3.2.10 (S)-styrene oxide + H2O the enzyme enantioselective and preferentially hydrolyzes (S)-styrene oxide Trichoderma reesei QM9414 (S)-styrene glycol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.10 23 50 highly active at temperatures ranging from 23°C to 50°C Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.3.2.10 37
-
 the enzyme conserves 80% of its activity for at least 20 min Trichoderma reesei
3.3.2.10 50
-
 activity decreases by 40% in the first 10 min, and 60% of the activity is preserved until 20 min of incubation Trichoderma reesei
3.3.2.10 70
-
 t1/2: 7.4 min Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.10 265
-
 (S)-styrene oxide pH 7.0, 37°C Trichoderma reesei
3.3.2.10 336
-
 (R)-styrene oxide pH 7.0, 37°C Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 7.2
-
-
 Trichoderma reesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.3.2.10 6 8.2 pH 6.0: about 55% of maximal activity, pH 8.2: about 40% of maximal activity Trichoderma reesei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.3.2.10 15.6
-
 (R)-styrene oxide pH 7.0, 37°C Trichoderma reesei
3.3.2.10 88.3
-
 (S)-styrene oxide pH 7.0, 37°C Trichoderma reesei