Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hu, X.; Zhang, P.; Miao, M.; Zhang, T.; Jiang, B.
    Development of a recombinant D-mannose isomerase and its characterizations for D-mannose synthesis (2016), Int. J. Biol. Macromol., 89, 328-335 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.7 synthesis expression in Bacillus subtilis leads to secretion of enzyme. Under optimum conditions, production of D-mannose using crude enzyme reaches about 150 g/l with approximately 25% turnover yield Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.7 expression in Bacillus subtilis Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.7 86.4
-
 D-mannose pH 7.0, 45°C Escherichia coli
5.3.1.7 203.7
-
 D-fructose pH 7.0, 45°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.7 274500
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.7 Escherichia coli AJH12524
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.7 D-fructose
-
 Escherichia coli D-mannose
-
 r
5.3.1.7 D-Mannose
-
 Escherichia coli D-Fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.7 hexamer 6 * 45000, calculated from sequence, 6 * 45800, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.3.1.7 yihS
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.7 45
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.7 61.4
-
 melting temperature Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.7 27.7
-
 D-fructose pH 7.0, 45°C Escherichia coli
5.3.1.7 40.9
-
 D-mannose pH 7.0, 45°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.7 7
-
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.7 0.136
-
 D-fructose pH 7.0, 45°C Escherichia coli
5.3.1.7 0.473
-
 D-mannose pH 7.0, 45°C Escherichia coli