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Literature summary extracted from

  • Abbassi, S.J.; Vishwakarma, R.K.; Patel, P.; Kumari, U.; Khan, B.M.
    Bacopa monniera recombinant mevalonate diphosphate decarboxylase Biochemical characterization (2015), Int. J. Biol. Macromol., 79, 661-668 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.33 expression in Escherichia coli Bacopa monnieri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.33 0.144
-
 (R)-5-diphosphomevalonate pH 8.0, temperature not specified in the publication Bacopa monnieri
4.1.1.33 1.386
-
 ATP pH 8.0, temperature not specified in the publication Bacopa monnieri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.33 Mg2+ optimum activity in presence of Mg2+ Bacopa monnieri
4.1.1.33 additional information divalent cation required Bacopa monnieri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.33 47000
-
 MALDI-TOF Bacopa monnieri
4.1.1.33 94000
-
 MALDI-TOF Bacopa monnieri

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.33 Bacopa monnieri A0A0F6WBJ2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.33 36.7
-
 pH 8.0, temperature not specified in the publication Bacopa monnieri

Storage Stability

EC Number Storage Stability Organism
4.1.1.33 20°C, pH 7.0, half-life 68 h Bacopa monnieri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.33 ATP + (R)-5-diphosphomevalonate
-
 Bacopa monnieri ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.33 dimer 1 * 47000, calculated from sequence and SDS-PAGE, and monomer Bacopa monnieri
4.1.1.33 monomer 1 * 47000, calculated from sequence and SDS-PAGE, and dimer Bacopa monnieri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.33 45
-
-
 Bacopa monnieri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.33 30
-
 ATP pH 8.0, temperature not specified in the publication Bacopa monnieri
4.1.1.33 40
-
 (R)-5-diphosphomevalonate pH 8.0, temperature not specified in the publication Bacopa monnieri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.33 8
-
-
 Bacopa monnieri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.33 Bacopa monnieri calculated from sequence
-
 6.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.33 22
-
 ATP pH 8.0, temperature not specified in the publication Bacopa monnieri
4.1.1.33 277
-
 (R)-5-diphosphomevalonate pH 8.0, temperature not specified in the publication Bacopa monnieri