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Literature summary extracted from

  • Li, X.; Zhao, J.; Fu, J.; Pan, Y.; Li, D.
    Sequence analysis and biochemical properties of an acidophilic and hyperthermophilic amylopullulanase from Thermofilum pendens (2018), Int. J. Biol. Macromol., 114, 235-243 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 heterologously expressed in Escherichia coli Thermofilum pendens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 additional information
-
 pullulan Km-value: 0.08 mg/ml, pH 3.5, 95°C Thermofilum pendens
3.2.1.41 additional information
-
 amylose Km-value: 0.71 mg/ml, pH 3.5, 95°C Thermofilum pendens
3.2.1.41 additional information
-
 amylopectin Km-value: 3.95 mg/ml, pH 3.5, 95°C Thermofilum pendens
3.2.1.41 additional information
-
 starch Km-value: 7.29 mg/ml, pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.25
-
 alpha-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.71
-
 gamma-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 5.16
-
 beta-cyclodextrin pH 3.5, 95°C Thermofilum pendens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 65500
-
 SDS-PAGE Thermofilum pendens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Thermofilum pendens A1RWP0
-
-
3.2.1.41 Thermofilum pendens DSM 2475 A1RWP0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 Thermofilum pendens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 alpha-cyclodextrin + H2O
-
 Thermofilum pendens ?
-
 ?
3.2.1.41 amylopectin + H2O the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C Thermofilum pendens ?
-
 ?
3.2.1.41 amylopectin + H2O the enzyme prefers to debranch the DP6-12 side chains of amylopectin at pH 4.5 and 100°C Thermofilum pendens DSM 2475 ?
-
 ?
3.2.1.41 amylose + H2O
-
 Thermofilum pendens ?
-
 ?
3.2.1.41 amylose + H2O
-
 Thermofilum pendens DSM 2475 ?
-
 ?
3.2.1.41 beta-cyclodextrin + H2O in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced Thermofilum pendens ?
-
 ?
3.2.1.41 beta-cyclodextrin + H2O in the initial stages, the ring-opening reactions of 6-O-glucosyl-beta-cyclodextrin, 6-O-maltosyl-beta-cyclodextrin and the debranching reactions of 6-O-maltooctaosyl-beta-cyclodextrin are firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced Thermofilum pendens DSM 2475 ?
-
 ?
3.2.1.41 gamma-cyclodextrin + H2O in the initial stages, the ring-opening reactions of gamma-cyclodextrin is firstly catalyzed. In the subsequent reactions, a serial of maltooligosaccharides are produced Thermofilum pendens ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Thermofilum pendens ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Thermofilum pendens DSM 2475 ?
-
 ?
3.2.1.41 starch + H2O
-
 Thermofilum pendens ?
-
 ?
3.2.1.41 starch + H2O
-
 Thermofilum pendens DSM 2475 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 65500, SDS-PAGE Thermofilum pendens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 amylopullulanase
-
 Thermofilum pendens
3.2.1.41 TPApu
-
 Thermofilum pendens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 95 100
-
 Thermofilum pendens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 0.03
-
 gamma-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.09
-
 alpha-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.11
-
 beta-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.11
-
 amylose pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.73
-
 pullulan pH 3.5, 95°C Thermofilum pendens
3.2.1.41 1.67
-
 starch pH 3.5, 95°C Thermofilum pendens
3.2.1.41 1.69
-
 amylopectin pH 3.5, 95°C Thermofilum pendens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 3.5
-
-
 Thermofilum pendens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.41 0.02
-
 beta-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.04
-
 gamma-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.043
-
 amylopectin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.16
-
 amylose pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.23
-
 starch pH 3.5, 95°C Thermofilum pendens
3.2.1.41 0.36
-
 alpha-cyclodextrin pH 3.5, 95°C Thermofilum pendens
3.2.1.41 8.79
-
 pullulan pH 3.5, 95°C Thermofilum pendens