Any feedback?
Literature summary extracted from
- Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase (2015), Front. Plant Sci., 6, 446 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.30 | isozyme RGP1, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
| 5.4.99.30 | isozyme RGP2, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
| 5.4.99.30 | isozyme RGP3, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
| 5.4.99.30 | isozyme RGP4, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.30 | D110A | site-directed mutagenesis | Brachypodium distachyon |
| 5.4.99.30 | D111A | site-directed mutagenesis | Brachypodium distachyon |
| 5.4.99.30 | D112A | site-directed mutagenesis | Brachypodium distachyon |
| 5.4.99.30 | H273A | site-directed mutagenesis | Brachypodium distachyon |
| 5.4.99.30 | additional information | specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature | Brachypodium distachyon |
| 5.4.99.30 | additional information | specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions | Brachypodium distachyon |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.30 | UDP-beta-L-arabinofuranose | Brachypodium distachyon | - |
UDP-beta-L-arabinopyranose | - |
? |  |
| 5.4.99.30 | UDP-beta-L-arabinofuranose | Brachypodium distachyon Bd21 | - |
UDP-beta-L-arabinopyranose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.30 | Brachypodium distachyon | I1GQE9 | - |
- |
| 5.4.99.30 | Brachypodium distachyon | I1GSJ4 | - |
- |
| 5.4.99.30 | Brachypodium distachyon | I1HRV4 | - |
- |
| 5.4.99.30 | Brachypodium distachyon | I1J2W9 | - |
- |
| 5.4.99.30 | Brachypodium distachyon Bd21 | I1J2W9 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 5.4.99.30 | glycoprotein | the enzyme is a reversibly glycosylated protein | Brachypodium distachyon |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.30 | leaf | - |
Brachypodium distachyon | - |
| 5.4.99.30 | leaf sheath | - |
Brachypodium distachyon | - |
| 5.4.99.30 | additional information | isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes | Brachypodium distachyon | - |
| 5.4.99.30 | additional information | RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview | Brachypodium distachyon | - |
| 5.4.99.30 | seedling | - |
Brachypodium distachyon | - |
| 5.4.99.30 | stem | - |
Brachypodium distachyon | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.30 | UDP-beta-L-arabinofuranose | - |
Brachypodium distachyon | UDP-beta-L-arabinopyranose | - |
? | |
| 5.4.99.30 | UDP-beta-L-arabinofuranose | - |
Brachypodium distachyon Bd21 | UDP-beta-L-arabinopyranose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.30 | BdRGP1 | - |
Brachypodium distachyon |
| 5.4.99.30 | Bradi1g15050 | locus name | Brachypodium distachyon |
| 5.4.99.30 | Bradi1g21990 | locus name | Brachypodium distachyon |
| 5.4.99.30 | Bradi2g50660 | locus name | Brachypodium distachyon |
| 5.4.99.30 | Bradi5g24850 | locus name | Brachypodium distachyon |
| 5.4.99.30 | LOC100822188 | locus name | Brachypodium distachyon |
| 5.4.99.30 | LOC100828998 | locus name | Brachypodium distachyon |
| 5.4.99.30 | LOC100836426 | locus name | Brachypodium distachyon |
| 5.4.99.30 | LOC100838265 | locus name | Brachypodium distachyon |
| 5.4.99.30 | RGP1 | - |
Brachypodium distachyon |
| 5.4.99.30 | RGP2 | - |
Brachypodium distachyon |
| 5.4.99.30 | RGP3 | - |
Brachypodium distachyon |
| 5.4.99.30 | RGP4 | - |
Brachypodium distachyon |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.30 | malfunction | RNAi mutants of isozyme BdRGP1 result in large alterations in cell wall carbohydrate composition with significant decreases in cell wall arabinose content but with minimal change in plant stature. Real-time PCR analysis indicates that gene expression levels for BdRGP1, BdRGP2, and BdRGP3 are reduced in RNAi-RGP1 plants to 15-20%of controls. Cell wall arabinose content is reduced by 23-51%of control levels. No alterations in cell wall xylose and glucose content are observed. Corresponding decreases in cell wall ferulic acid and ferulic acid-dimers are observed. Additionally, cell wall 4-coumarates are decreased. The cell wall decrease in 4-coumarates corresponds to arabinose-coupled 4-coumarates. Xylanase-mediated digestibility of RNAi-RGP1 Brachypodium cell walls results in a near 2fold increase of released total carbohydrate. Cellulolytic hydrolysis of cell wall material is inhibited in leaves of RNAi-RGP1 mutants | Brachypodium distachyon |
| 5.4.99.30 | physiological function | the enzyme is involved in cell wall carbohydrate biosynthesis, the cell wall composition has a complex effect on digestibility of grass plants | Brachypodium distachyon |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
