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Literature summary extracted from

  • Peng, C.; Wang, Q.; Lu, D.; Han, W.; Li, F.
    A novel bifunctional endolytic alginate lyase with variable alginate-degrading modes and versatile monosaccharide-producing properties (2018), Front. Microbiol., 9, 167 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression in Escherichia coli Flammeovirga sp. MY04

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.3 T282N deletion of the noncatalytic region, 1.5fold increase in specific activity compared to wild-type Flammeovirga sp. MY04

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 alginate Km value of wild-type 0.135 mg/ml, of mutant T282N 0.158 mg/ml, pH 6.0, 40°C Flammeovirga sp. MY04
4.2.2.3 additional information
-
 poly(alpha-(1,4)-L-guluronate) Km value of wild-type 0.706 mg/ml, of mutant T282N 1.224 mg/ml, pH 6.0, 40°C Flammeovirga sp. MY04
4.2.2.3 additional information
-
 poly(beta-(1,4)-D-mannuronate) Km value of wild-type 2.845 mg/ml, of mutant T282N 0.392 mg/ml, pH 6.0, 40°C Flammeovirga sp. MY04

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Flammeovirga sp. MY04 A0A1B1FUS6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 purification using Ni-NTA column Flammeovirga sp. MY04

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 2025
-
 wild-type, pH 6.0, 40°C Flammeovirga sp. MY04
4.2.2.3 2920
-
 mutant T282N, pH 6.0, 40°C Flammeovirga sp. MY04

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Flammeovirga sp. MY04 unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 poly(alpha-(1,4)-L-guluronate) reaction of EC 4.2.2.11, about 15% of the activity with poly(beta-(1,4)-D-mannuronate) Flammeovirga sp. MY04 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate)
-
 ?
4.2.2.3 poly(beta-(1,4)-D-mannuronate)
-
 Flammeovirga sp. MY04 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate)
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 60580, calculated from sequence Flammeovirga sp. MY04

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 Aly2
-
 Flammeovirga sp. MY04
4.2.2.3 MY04_2544
-
 Flammeovirga sp. MY04

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40
-
 wild-type, 2 h, less than 30% residual activity. Mutant T282N, 8 h, 50% residual activity Flammeovirga sp. MY04

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Flammeovirga sp. MY04 calculated from sequence
-
 9.1