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Literature summary extracted from

  • Haraguchi, K.
    Difructose dianhydride III producing inulin fructotransferase from Microbacterium sp. S48-1 (2015), Food Biotechnol., 29, 156-165 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.18 5
-
 inulin pH 6.0, 60°C Microbacterium sp. S48-1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.18 extracellular
-
 Microbacterium sp. S48-1
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.18 46000
-
 gel filtration Microbacterium sp. S48-1

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.18 Microbacterium sp. S48-1
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.18 culture fluid
-
 Microbacterium sp. S48-1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.18 106
-
 substrate inulin, pH 6.0, 60°C Microbacterium sp. S48-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.18 beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
-
 Microbacterium sp. S48-1 di-D-fructofuranose 1,2':2,3' dianhydride + 1-kestose
-
 ?
4.2.2.18 inulin
-
 Microbacterium sp. S48-1 di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
 ?
4.2.2.18 additional information smallest oligosaccharide accepted is nystose Microbacterium sp. S48-1 ?
-
 ?
4.2.2.18 nystose
-
 Microbacterium sp. S48-1 di-D-fructofuranose 1,2':2,3'-dianhydride + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.18 monomer 1 * 45000, SDS-PAGE Microbacterium sp. S48-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.18 60
-
-
 Microbacterium sp. S48-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.18 65
-
 30 min, about 80% residual activtiy. Rapid loss of activity above 65°C Microbacterium sp. S48-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.18 6
-
-
 Microbacterium sp. S48-1