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Literature summary extracted from

  • North, R.A.; Watson, A.J.; Pearce, F.G.; Muscroft-Taylor, A.C.; Friemann, R.; Fairbanks, A.J.; Dobson, R.C.
    Structure and inhibition of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus (2016), FEBS Lett., 590, 4414-4428 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.3 ligand-free and (2R)-sialic acid alditol-bound enzyme, hanging drop vapor diffusion method, using Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 (2R)-sialic acid alditol
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 3.2
-
 N-acetylneuraminate at 30°C, pH not specified in the publication Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 129
-
 calculated from amino acid sequence Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.3 N-acetylneuraminate Staphylococcus aureus
-
 N-acetyl-D-mannosamine + pyruvate
-
 ?
4.1.3.3 N-acetylneuraminate Staphylococcus aureus NCTC 8325
-
 N-acetyl-D-mannosamine + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Staphylococcus aureus Q2G160
-
-
4.1.3.3 Staphylococcus aureus NCTC 8325 Q2G160
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-acetylneuraminate
-
 Staphylococcus aureus N-acetyl-D-mannosamine + pyruvate
-
 ?
4.1.3.3 N-acetylneuraminate
-
 Staphylococcus aureus NCTC 8325 N-acetyl-D-mannosamine + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.3 homotetramer 4 * 33000, calculated from amino acid sequence Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.3 22.1
-
 N-acetylneuraminate at 30°C, pH not specified in the publication Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.3 0.39
-
 (2R)-sialic acid alditol at 30°C, pH not specified in the publication Staphylococcus aureus