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Literature summary extracted from

  • Bae, E.; Kim, H.E.; Koh, E.; Kim, K.S.
    Phosphoglucomutase1 is necessary for sustained cell growth under repetitive glucose depletion (2014), FEBS Lett., 588, 3074-3080 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.2 alpha-D-Glucose 1,6-bisphosphate dependent on Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expressed in Escherichia coli BL21 and Rosetta(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.2 T556A the mutant shows about wild type activity Homo sapiens
5.4.2.2 T556E the mutant shows about wild type activity Homo sapiens
5.4.2.2 T559A the mutant shows about wild type activity Homo sapiens
5.4.2.2 T559E the mutant shows about wild type activity Homo sapiens
5.4.2.2 T562A the mutant shows slightly reduced activity compared to the wild type enzyme Homo sapiens
5.4.2.2 T562E the mutant shows strongly reduced activity compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.2 additional information enzyme with a C-terminus 6xHis tag completely loses activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate Homo sapiens
-
 D-glucose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Homo sapiens P36871
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 Ni-NTA agarose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.2 HEK-293T cell
-
 Homo sapiens
-
5.4.2.2 HeLa cell
-
 Homo sapiens
-
5.4.2.2 MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Homo sapiens D-glucose 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 PGM1
-
 Homo sapiens
5.4.2.2 phosphoglucomutase1
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
5.4.2.2 malfunction enzyme depletion is associated with declined cellular glycogen content and decreased rates of glycogenolysis and glycogenesis. Furthermore, enzyme depletion suppresses cell proliferation under long-term repetitive glucose depletion Homo sapiens
5.4.2.2 physiological function the enzyme is necessary for sustained cell growth under repetitive glucose depletion Homo sapiens